Virginia Tech
    • Log in
    View Item 
    •   VTechWorks Home
    • ETDs: Virginia Tech Electronic Theses and Dissertations
    • Doctoral Dissertations
    • View Item
    •   VTechWorks Home
    • ETDs: Virginia Tech Electronic Theses and Dissertations
    • Doctoral Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Molecular cloning, characterization, and expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase gene from tomato

    Thumbnail
    View/Open
    LD5655.V856_1990.P375.pdf (7.414Mb)
    Downloads: 26
    Date
    1990
    Author
    Park, Hee-Sung
    Metadata
    Show full item record
    Abstract
    In plants, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC 1.1.1.34) is a key enzyme regulating biosynthesis of phytosterols, plant growth regulators, carotenoids, antimicrobial defense compounds, and numerous other isoprenoids. To initiate molecular studies of HMGR in relation to defense responses in plants, we utilized yeast HMGR cDNA sequences to isolate tomato genomic sequences encoding HMGR. The nucleic acid sequence and gene structure was determined. The tomato HMGR gene (HMG2) contains four exons separated by three introns and encodes a polypeptide of 602 amino acid residues (about 64,714 Da). Two membrane-spanning regions are contained in the NH₂-terminus of the HMGR polypeptide. The COOH-terminus shares significant homology with HMGR sequences from different species. Genomic Southern hybridization analyses reveals that tomato contains 3 to 4 HMGR genes. The HMGz2 gene cross-hybridizes to mRNA of about 2.7 kb which is highly induced in tomato cells treated with fungal elicitors and in stems, leaves, or roots stressed by wounding suggesting that the HMGz2 is a defense-related gene in tomato. Hybridization with a gene specific probe indicates that the HMG2 gene is induced specifically during defense responses and is distinct from the gene(s) expressed during fruit development and ripening.
    URI
    http://hdl.handle.net/10919/37402
    Collections
    • Doctoral Dissertations [15775]

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us
     

     

    VTechWorks

    AboutPoliciesHelp

    Browse

    All of VTechWorksCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Log inRegister

    Statistics

    View Usage Statistics

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us