Changes in macrophage functions and gene expression during tumor growth
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Functions and phenotypes change in macrophages (MÃ¸,) during tumor growth. Although analyzing functional and phenotypic changes are important in understanding the mechanism of tumor-induced immunosuppression, it is necessary to look beneath the surface and expose the mechanisms behind these changes. Flow cytometrically isolated Mac-1 +, -2+, or -3+ MÃ¸, showed that although both normal host and tumor-bearing host (TBH) Mac-2+ MÃ¸, were the primary source of prostaglandin E2, no specific TBH suppressor MÃ¸, could be identified. To determine if normal host and TBH MÃ¸, respond to in vitro activating agents differently, normal host and TBH MÃ¸, were treated with lipopolysaccharide. Functional, phenotypic, and molecular changes were observed in the lipopolysaccharide-treated M4J. Three- and 24-h lipopolysaccharide treatment reduced TBH MÃ¸,-mediated suppression, while only 24-h lipopolysaccharide treatment reduced it in normal host MÃ¸,. Prolonged adherence, which induces MÃ¸, differentiation, increases the number of Mac-2+ TBH MÃ¸,. Tumor growth causes an increase of MÃ¸, in the S and G2/M phases of the cell cycle. Lipopolysaccharide and adherence increase the number of normal host MÃ¸, in Sand G2/M; however, these same treatments reduce the nu mber of TBH MÃ¸, in these same phases.
- Doctoral Dissertations