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dc.contributor.authorMadurawe, Rapti D.en_US
dc.date.accessioned2014-03-14T21:20:47Z
dc.date.available2014-03-14T21:20:47Z
dc.date.issued1990-04-05en_US
dc.identifier.otheretd-10122005-134402en_US
dc.identifier.urihttp://hdl.handle.net/10919/39744
dc.description.abstract

This study focuses on the utilization of metal-dependent monoclonal antibodies for large-scale chromatography and addresses an aspect that has been cited to lower immunosorbent performance, namely "orientation" of antibodies on matrices. The antibodies used in this study, the "EDTAdependent" 7D7BlO and the "Ca2+ -dependent" HPC4 are directed against human Protein C (PC).

The 7D7BI0 antibody was characterized in terms of its metaldependency and specificity. The region of PC (epitope) recognized by 7D7BlO was identified as the first 15 residues in the NH2-terminal. Immunosorbents made with 7D7BI0 provided highly pure and functional PC.

The "orientation" of the antibodies on matrices was addressed in two ways. In the first approach, performance of immunosorbents coupled through carbohydrate moieties were compared with immunosorbents coupled through peptide regions. Coupling via carbohydrate linkages, which is generally believed to be Fc-directed, did not have any advantage in terms of efficiency and recovery over coupling via peptide.

en_US
dc.format.mediumBTDen_US
dc.publisherVirginia Techen_US
dc.relation.haspartLD5655.V856_1990.M349.pdfen_US
dc.subjectMonoclonal antibodies Research.en_US
dc.subjectChromatographic analysis.en_US
dc.subject.lccLD5655.V856 1990.M349en_US
dc.titleOptimization of metal dependent antibodies for chromatographyen_US
dc.typeDissertationen_US
dc.contributor.departmentChemical Engineeringen_US
dc.description.degreePh. D.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineChemical Engineeringen_US
dc.contributor.committeechairVelander, William H.en_US
dc.contributor.committeememberDrohan, William N.en_US
dc.contributor.committeememberConger, William L.en_US
dc.contributor.committeememberOrthner, C. L.en_US
dc.contributor.committeememberWilkins, Tracy D.en_US
dc.contributor.committeememberMichelsen, Donald L.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10122005-134402/en_US
dc.date.sdate2005-10-12en_US
dc.date.rdate2005-10-12
dc.date.adate2005-10-12en_US


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