Biodegradation of pesticide and indolic compounds under methanogenic conditions
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Degradability of atrazine, cyanazine, and dicamba under methanogenic conditions was evaluated using serum bottle microcosms containing wetland soil inocula obtained from three different sites. Pesticides were monitored by high-performance liquid chromatography (HPLC) and the production of methane was measured with a gas chromatograph (GC). Dicamba was the most susceptible to degradation in the microcosms, followed by cyanazine. Atrazine was not degraded in the wetland soils. A dicamba-degrading methanogenic consortium was enriched from one of the initial wetland soil microcosms (Lawnes). Dicamba degradation was further examined using this consortium. Net methane production suggested that the aromatic ring was not degraded. Rates of dicamba degradation were enhanced with addition of O. 2 % yeast extract.
Dicamba degradation was accomplished within 4 days compared to 22 days without yeast extract addition. The inability of the consortium to degrade the benzenoid ring was confirmed when no 14C02 was produced upon addition of [U-14C]dicamba to the cultures. Analysis of culture filtrate by HPLC revealed the presence of a possible metabolite that was aromatic in character.
- Doctoral Dissertations