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dc.contributor.authorBramble, Lisa Anneen_US
dc.date.accessioned2014-03-14T21:31:03Z
dc.date.available2014-03-14T21:31:03Z
dc.date.issued1990-01-18en_US
dc.identifier.otheretd-03122009-040409en_US
dc.identifier.urihttp://hdl.handle.net/10919/41443
dc.description.abstractShort-term toxicity tests utilizing mitochondrial and microsomal metabolism were developed and applied to a series of eight quinones. In the mitochondrial assay, the degree to which test compounds inhibited mitochondrial respiration varied from an EC50 of 9 μM to l25 μM. In the microsomal assay, the maximum percent increase over control oxygen consumption rates elicited by the quinones ranged from eight percent to 837 percent. The ability of the compounds to stimulate microsomal oxygen uptake reflects their capability to redox cycle and form reactive oxygen species. Experiments were conducted to evaluate the relationship between the rate of quinone redox cycling and the extent of microsomal lipid peroxidation, a possible toxic insult associated with reactive oxygen species. Results of the mitochondrial and microsomal assays were statistically correlated with several quinone physicochemical parameters and qualitatively compared to reduction potential. The biological response observed in both test systems appeared to be most strongly influenced by the reduction potential of the quinone and biomechanisms of action were suggested based on this relationship. To assess the ability of the mitochondrial and microsomal assays to indicate toxicity of the quinonoid compounds, results were statistically correlated with literature-derived toxicity data. It was concluded that the mitochondrial assay appears to be a valid indicator of acute toxicity, while the microsomal assay better portends the potential for chronic toxicity.en_US
dc.format.mediumBTDen_US
dc.publisherVirginia Techen_US
dc.relation.haspartLD5655.V855_1990.B725.pdfen_US
dc.subjectMicrosomesen_US
dc.subject.lccLD5655.V855 1990.B725en_US
dc.titleUtilization of mitochondrial and microsomal metabolism for the assessment of toxicityen_US
dc.typeThesisen_US
dc.contributor.departmentEnvironmental Sciences and Engineeringen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineEnvironmental Sciences and Engineeringen_US
dc.contributor.committeechairBoardman, Gregory D.en_US
dc.contributor.committeememberBevan, David R.en_US
dc.contributor.committeememberDietrich, Andrea M.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-03122009-040409/en_US
dc.date.sdate2009-03-12en_US
dc.date.rdate2009-03-12
dc.date.adate2009-03-12en_US


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