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dc.contributor.authorRichter, Kevinen_US
dc.date.accessioned2014-03-14T21:32:47Z
dc.date.available2014-03-14T21:32:47Z
dc.date.issued2012-02-16en_US
dc.identifier.otheretd-04062012-133842en_US
dc.identifier.urihttp://hdl.handle.net/10919/41893
dc.description.abstractThe demand for biodegradable packaging materials as an alternative to synthetic ones to reduce environmental cost has seen an increase in recent years. In addition, functionalizing the packaging film to provide specific advantages like antimicrobial properties has yet to be explored thoroughly. This study considers adding antimicrobial agents to improve the quality and safety of actively packaged fresh produce using an antimicrobial enzyme (lysozyme) immobilized on a biopolymer based packaging film (corn-zein). The developed packaging material is aimed as an active biodegradable packaging to reduce bacterial contamination on the surface of fresh organic produce, specifically tomatoes. The study uses glutaraldehyde and glyoxal as binding agents to immobilize the enzyme on the packaging film. The effect of concentration of glutaraldehyde and glyoxal on the controlled release of the enzyme was studied. Concentrations of 0.1 and 0.2 g/g lysozyme: cross linking agent had controlled release properties. However, concentrations of 0 or 0.05 are about 20-30% more effective at inactivating bacteria. Antimicrobial activity in the constructed zein films are also tested against selected pathogens (Salmonella Newport and Listeria monocytogenes). Developed zein based film is tested against inoculated tomatoes to determine the efficacy of the films in reducing the pathogen population. The inoculated tomatoes are stored at room temperature over a storage period of one week. The film was able to reduce Listeria monocytogenes population by three logs but was unable to reduce the population of Salmonella Newport.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartRichter_K_T_2012.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectGlyoxalen_US
dc.subjectGlutaraldehydeen_US
dc.subjectLysozymeen_US
dc.subjectFilmsen_US
dc.subjectZeinen_US
dc.subjectActive Packagingen_US
dc.subjectTomatoesen_US
dc.subjectCross linkingen_US
dc.subjectFood Safetyen_US
dc.subjectListeriaen_US
dc.subjectSalmonellaen_US
dc.titleEffect of concentration of glutaraldehyde and glyoxal on binding lysozyme to zein based films to control foodborne pathogens in tomatoesen_US
dc.typeThesisen_US
dc.contributor.departmentBiological Systems Engineeringen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
dc.contributor.committeechairMallikarjunan, Parameswarakumaren_US
dc.contributor.committeememberKrometis, Leigh Anne Henryen_US
dc.contributor.committeememberWilliams, Robert C.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04062012-133842/en_US
dc.date.sdate2012-04-06en_US
dc.date.rdate2012-05-03
dc.date.adate2012-05-03en_US


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