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dc.contributor.authorAllevi, Richard Paulen_US
dc.date.accessioned2014-03-14T21:33:30Z
dc.date.available2014-03-14T21:33:30Z
dc.date.issued2012-03-26en_US
dc.identifier.otheretd-04092012-194303en_US
dc.identifier.urihttp://hdl.handle.net/10919/42011
dc.description.abstractIn Virginia, over one million households rely on private water supplies (e.g. well, spring, cistern). Previous literature acknowledges bacterial contamination in private water supplies as a significant public health concern in the United States. The present study tested private wells and springs in 20 Virginia counties for total coliforms (TC) and E. coli (EC) along with a suite of chemical contaminants. Sample collection was organized by the Virginia Household Water Quality Program (VAHWQP), a Virginia Cooperative Extension effort managed by faculty in the Biological Systems Engineering Department. Microbial and chemical source tracking were used to identify possible sources of contamination. A logistic regression was employed to investigate potential correlations between TC contamination and chemical parameters (e.g. NO3-, turbidity) as well as homeowner provided survey data describing system characteristics and perceived water quality. TC and EC contamination were quantified via the Colilert (www.idexx.com) defined substrate method for most probable number (MPN) of EC and TC per 100 mL of water. Of the 538 samples collected, 41% (n=221) were positive for TC and 10% (n=53) for EC. Chemical parameters were not statistically predictive of microbial contamination. Well depth, water treatment, and farm location proximate to the water supply were factors in a regression model that predicted presence/absence of TC with 74% accuracy. Microbial and chemical source tracking techniques (Polymerase Chain Reaction (PCR) and fluorometry, respectively) identified 4 of 26 samples as likely contaminated with human wastewater. Application of these source-tracking analyses on a larger scale will prove useful in defining remediation strategies.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartAllevi_RP_T_2012.PDFen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectCooperative Extensionen_US
dc.subjectWellen_US
dc.subjectMicrobial Source Trackingen_US
dc.subjectPCRen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectOptical Birghtenersen_US
dc.subjectIndicator Organismsen_US
dc.subjectPrivate Drinking Wateren_US
dc.titleQuantifying Potential Sources of Microbial Contamination in Household Drinking Water Samplesen_US
dc.typeThesisen_US
dc.contributor.departmentBiological Systems Engineeringen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineBiological Systems Engineeringen_US
dc.contributor.committeechairKrometis, Leigh Anne Henryen_US
dc.contributor.committeememberHagedorn, Charles, IIIen_US
dc.contributor.committeememberBenham, Brian L.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04092012-194303/en_US
dc.date.sdate2012-04-09en_US
dc.date.rdate2012-05-30
dc.date.adate2012-05-30en_US


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