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dc.contributor.authorSumners, Lindsay Harten_US
dc.date.accessioned2014-03-14T21:38:53Z
dc.date.available2014-03-14T21:38:53Z
dc.date.issued2011-06-06en_US
dc.identifier.otheretd-06202011-123953en_US
dc.identifier.urihttp://hdl.handle.net/10919/43370
dc.description.abstractChickens genetically selected for low (LA) or high (HA) antibody response to sheep red blood cells (SRBC) displayed a correlated change in major histocompatibility complex (MHC), so that LA chickens were 96% B13 and HA chickens were 96% B21. During a clinical outbreak of necrotic enteritis, B21B21 genotypes experienced significantly less mortality (6% vs. 13 %) compared to B13B13 genotypes. A study was carried out to assess immunological differences between LA and HA lines during exposure to Clostridium perfringens. In Experiment 1, chickens were orally gavaged with a low (10^7 CFU/mL) or high (10^9 CFU/mL) dose of C. perfringens. In Experiment 2, chickens were orally gavaged with live coccidia oocysts on experiment d 1, followed by 107 CFU/mL C. perfringens on d 5. Unfortunately, establishment of necrotic enteritis infection was unsuccessful in both experiments as evidenced by lack of significant intestinal lesions, as well as no negative effect on bird performance. In an ex vivo study, peripheral blood mononuclear cells (PBMCs) were isolated from each genetic line, cultured, stimulated with LPS (4 h), and exposed to varying concentrations of C. perfringens α- toxin (1, 10, 100, 1000 U/L) for 2 and 4 h. Evaluation of cellular proliferation, percent cytotoxicity and immunological gene expression was carried out in a variety of experiments. Genetic lines were found to be highly divergent in all analyses.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartSumners_LH_T_2011_Corrected.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectimmune responseen_US
dc.subjectgenetic resistanceen_US
dc.subjectnecrotic enteritisen_US
dc.subjectClostridium perfringensen_US
dc.subjectchickenen_US
dc.titleImmunological Response to Clostridium perfringens in Two Genetically Divergent Lines of Chickens as Influenced by Major Histocompatibility Complex (MHC) Genotypeen_US
dc.typeThesisen_US
dc.contributor.departmentAnimal and Poultry Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineAnimal and Poultry Sciencesen_US
dc.contributor.committeechairDalloul, Rami Alien_US
dc.contributor.committeememberMcElroy, Audrey P.en_US
dc.contributor.committeememberWong, Eric A.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06202011-123953/en_US
dc.date.sdate2011-06-20en_US
dc.date.rdate2011-07-28
dc.date.adate2011-07-28en_US


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