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dc.contributor.authorKeller, Scott W.en_US
dc.date.accessioned2014-03-14T21:40:04Z
dc.date.available2014-03-14T21:40:04Z
dc.date.issued1995-01-05en_US
dc.identifier.otheretd-07102009-040603en_US
dc.identifier.urihttp://hdl.handle.net/10919/43643
dc.description.abstract

The objective of this study was to determine how hole diameter, channel length, test organism motility, concentration and aerosol exposure time affected microbiological contamination of sealed flexible pouches. Nickel microtubes with 10 μm and 20μm hole diameters and lengths of 5 mm and 10 mm were used in various combinations to create seal defects in 128 retortable pouches. A 119,911 cm3, exposure chamber was used to distribute an aerosol with a particle size of 2.68 μm, infected with motile and isogenically mutated nonmotile Pseudomonas fragi TM 849 in concentrations of 102 or 106 cells/mL. Fifteen and 30 minute aerosol exposure times were used. Six pouches tested positive for test organism growth after a 72 hour incubation period. Pouch contamination via microbial ingress was significant (P < .05) for test organism motility (motile) and concentration (106 cells/mL).

en_US
dc.format.mediumBTDen_US
dc.publisherVirginia Techen_US
dc.relation.haspartLD5655.V855_1995.K455.pdfen_US
dc.subjectcontaminationen_US
dc.subject.lccLD5655.V855 1995.K455en_US
dc.titleAerosol exposure biotesting for package integrity testingen_US
dc.typeThesisen_US
dc.contributor.departmentFood Science and Technologyen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineFood Science and Technologyen_US
dc.contributor.committeememberMarcy, Joseph E.en_US
dc.contributor.committeememberHackney, Cameron Rajen_US
dc.contributor.committeememberBlakistone, Barbara A.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07102009-040603/en_US
dc.date.sdate2009-07-10en_US
dc.date.rdate2009-07-10
dc.date.adate2009-07-10en_US


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