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dc.contributor.authorTasaki, Hiromien_US
dc.date.accessioned2014-03-14T21:41:05Z
dc.date.available2014-03-14T21:41:05Z
dc.date.issued2008-06-20en_US
dc.identifier.otheretd-07222008-112126en_US
dc.identifier.urihttp://hdl.handle.net/10919/43868
dc.description.abstractThe stage of maturity of seeds at harvest is an important factor that determines seed vigor. Separating seeds from a seed lot composed of many different stages of development can be difficult especially after maximum dry mass is attained. Separating seeds based on their physiological maturity is more challenging than sorting seeds based on their physical properties. Seeds may be non-destructively sorted using chlorophyll fluorescence (CF) as a marker of seed maturity. This study was conducted to test whether CF could be used to remove low vigor immature seeds from muskmelon (Cucumis melo L.'Top Mark') and tomato (Lycopersicum esculentum) seed lots. Light treatments were applied to determine whether the light environment during seed harvesting and processing could affect chlorophyll content and seed vigor. Seeds from nine stages of development were collected from 'TopMark'. Seeds from three stages of fruit development (red ripe, breaker, and mature green) were harvested from tomato cultivar Money Maker and two phytochrome mutants: phytochrome A mutant, fri-1 and phytochrome B mutant, tri-1. The SeedMaster Analyzer (Satake USA Inc., Houston Texas) was used to measure CF and to sort individual seeds according to CF levels. Immature tomato seeds and muskmelon, harvested from green fruits, had the highest CF (p>0.001). Contrary to the results obtained with the other tomato genotypes, the vigor of tri-1 did not change inversely with changing CF levels, rather, seeds with low CF had the same vigor as seeds with high CF. This result may suggest that the presence of phytochrome B exerts an inhibitory influence on vigor in tomato seeds, and that the persistent presence of chlorophyll during seed development does not affect vigor. The light treatments had no consistent effect on seed chlorophyll content or on vigor in either tomato or muskmelon.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartFinalThesis.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectvigoren_US
dc.subjectgerminationen_US
dc.subjectlighten_US
dc.subjecttomatoen_US
dc.subjectchlorophyllen_US
dc.subjectphytochromeen_US
dc.subjectmuskmelonen_US
dc.titleLight Effect on Seed Chlorophyll Content and Germination Performance of Tomato and Muskmelon Seedsen_US
dc.typeThesisen_US
dc.contributor.departmentHorticultureen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineHorticultureen_US
dc.contributor.committeechairWelbaum, Gregory E.en_US
dc.contributor.committeememberParrish, David J.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07222008-112126/en_US
dc.contributor.committeecochairGrene, Ruthen_US
dc.date.sdate2008-07-22en_US
dc.date.rdate2008-08-21
dc.date.adate2008-08-21en_US


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