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Comparison of packed and wide-bore, open-tubular gas chromatographic columns
Wide-bore, fused-silica capillary columns can be an excellent
replacement for packed columns in many cases. They can provide higher
surface inertness and thermal stability, as well as higher efficiency or
speed of analysis. Wide-bore, open-tubular (WBOT) columns with thick
films have higher sample capacities than conventional narrow-bore
capillary columns and can be used in unmodified packed-column gas
The majority of gas chromatographic analyses are still performed
using packed columns. Many chromatographers associate open-tubular
columns exclusively with split injection, often perceived as less
repeatable or quantitative. Also, many packed-column methods use
application-specific stationary phases which are not readily available on
capillary columns. WBOT columns with standard phases can often be
substituted due to higher available efficiencies. While several studies
have been published on qualitative differences, there is little comparative
quantitative data available on O.53â mm I.D. and packed columns.