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dc.contributor.authorArany, Catherine Beatriceen_US
dc.date.accessioned2014-03-14T21:51:29Z
dc.date.available2014-03-14T21:51:29Z
dc.date.issued1992-09-03en_US
dc.identifier.otheretd-12162009-020131en_US
dc.identifier.urihttp://hdl.handle.net/10919/46232
dc.description.abstract

Bifidobacterium spp. are anaerobic, Gram-positive, nonmotile bacteria that are a major component of intestinal microbiota. They are potential indicators of human fecal pollution in shellfish harvesting waters. In addition, Bifidobacterium spp. are used as supplements in dairy products. These bacteria are easily injured and it is important to have methods that will recover all cells (injured and uninjured) present in water and food samples. The objectives of this research were to develop a repair detection (RD) procedure to be used with VPI's anaerobic roll tube apparatus for improving the recovery of Bifidobacterium and to compare this method with existing enumeration methods.

Bifidobacterium adolescentis cells were injured by exposure to pond water. Cells were enumerated using a modification of Human Bifid Sorbitol Agar (MHBSA). MHBSA was modified by substituting phenyl red for bromocresol purple and adding methylene blue as an indicator of oxidation/reduction.

en_US
dc.format.mediumBTDen_US
dc.publisherVirginia Techen_US
dc.relation.haspartLD5655.V855_1992.A736.pdfen_US
dc.subjectBifidobacteriumen_US
dc.subject.lccLD5655.V855 1992.A736en_US
dc.titleEnhanced recovery of injured and noninjured cells of Bifidobacterium species from water and dairy productsen_US
dc.typeThesisen_US
dc.contributor.departmentFood Science and Technologyen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineFood Science and Technologyen_US
dc.contributor.committeechairHackney, Cameron Rajen_US
dc.contributor.committeememberPierson, Merle D.en_US
dc.contributor.committeememberEigel, William N. IIIen_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-12162009-020131/en_US
dc.date.sdate2009-12-16en_US
dc.date.rdate2009-12-16
dc.date.adate2009-12-16en_US


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