Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
Abstract
Three experiments were conducted to determine an efficient method of obtaining
oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal
oocyte recovery combined with in vitro maturation, fertilization, and culture
(IVMlIVFIIVC) can produce pre-implantation stage bovine embryos and also supply a
source of ova for gene microinjection. In Experiment 1, once- (IX) vs. twice-weekly
(2X) oocyte recovery frequencies were compared. No differences in recovery per session
were observed (IX = 6.8 vs. 2X = 6.3 oocytes/session; p>O.1 0). However, the 2X group
generated more oocytes on a weekly basis (IX = 6.8 vs. 2X = 12.6 oocytes/week;
p
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- Masters Theses [19617]