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    Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection

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    LD5655.V855_1994.G533.pdf (3.734Mb)
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    Date
    1994-05-15
    Author
    Gibbons, John R.
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    Abstract

    Three experiments were conducted to determine an efficient method of obtaining oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal oocyte recovery combined with in vitro maturation, fertilization, and culture (IVMlIVFIIVC) can produce pre-implantation stage bovine embryos and also supply a source of ova for gene microinjection. In Experiment 1, once- (IX) vs. twice-weekly (2X) oocyte recovery frequencies were compared. No differences in recovery per session were observed (IX = 6.8 vs. 2X = 6.3 oocytes/session; p>O.1 0). However, the 2X group generated more oocytes on a weekly basis (IX = 6.8 vs. 2X = 12.6 oocytes/week; pO.IO) In these experiments, twice-weekly follicular aspiration without exogenous FSH, was the best scheme of oocyte recovery. This program provided a source of consistent, high quality oocytes that responded favorable to the IVMIIVFIIVC system and microinjection.

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    http://hdl.handle.net/10919/46259
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    • Masters Theses [19617]

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