Virginia Tech
    • Log in
    View Item 
    •   VTechWorks Home
    • College of Engineering (COE)
    • School of Biomedical Engineering and Sciences
    • Scholarly Works, School of Biomedical Engineering and Sciences
    • View Item
    •   VTechWorks Home
    • College of Engineering (COE)
    • School of Biomedical Engineering and Sciences
    • Scholarly Works, School of Biomedical Engineering and Sciences
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Folate Conjugated Cellulose Nanocrystals Potentiate Irreversible Electroporation-induced Cytotoxicity for the Selective Treatment of Cancer Cells

    Thumbnail
    View/Open
    colacino_tcrt_2014.pdf (1.636Mb)
    Downloads: 1353
    Date
    2014-04-16
    Author
    Colacino, Katelyn R.
    Arena, Christopher B.
    Dong, Shuping
    Roman, Maren
    Davalos, Rafael V.
    Lee, Yong Woo
    Metadata
    Show full item record
    Abstract
    Cellulose nanocrystals are rod-shaped, crystalline nanoparticles that have shown prom- ise in a number of industrial applications for their unique chemical and physical properties. However, investigations of their abilities in the biomedical field are limited. The goal of this study is to show the potential use of folic acid-conjugated cellulose nanocrystals in the potentiation of irreversible electroporation-induced cell death in folate receptor (FR)-positive cancers. We optimized key pulse parameters including pulse duration, intensity, and incubation time with nanoparticles prior to electroporation. FR-positive cancer cells, KB and MDA-MB-468, were preincubated with cellulose nanocrystals (CNCs) conjugated with the targeting molecule folic acid (FA), 10 and 20 min respectively, prior to application of the optimized pulse electric field (PEF), 600 and 500 V/cm respectively. We have shown cellulose nanocrystals’ ability to potentiate a new technique for tumor ablation, irreversible electroporation. Pre-incubation with FA-conjugated CNCs (CNC-FA) has shown a significant increase in cytotoxicity induced by irreversible electroporation in FR-positive cancer cells, KB and MDA-MB-468. Non-targeted CNCs (CNC-COOH) did not potentiate IRE when preincubated at the same parameters as previously stated in these cell types. In addition, CNC-FA did not potentiate irreversible electroporation-induced cytotoxicity in a FR-negative cancer cell type, A549. Without changing irreversible electroporation parameters it is possible to increase the cytotoxic effect on FR-positive cancer cells by exploiting the specific binding of FA to the FR, while not causing further damage to FR-negative tissue.
    URI
    http://hdl.handle.net/10919/47959
    Collections
    • Destination Area: Global Systems Science (GSS) [612]
    • Scholarly Works, School of Biomedical Engineering and Sciences [135]
    • Strategic Growth Area: Economical and Sustainable Materials (ESM) [187]

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us
     

     

    VTechWorks

    AboutPoliciesHelp

    Browse

    All of VTechWorksCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Log inRegister

    Statistics

    View Usage Statistics

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us