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dc.contributorVirginia Techen_US
dc.contributor.authorStrobl, Jeannine S.en_US
dc.contributor.authorGoodwin, David G.en_US
dc.contributor.authorRzigalinski, Beverly A.en_US
dc.contributor.authorLindsay, David S.en_US
dc.date.accessioned2014-06-20T14:13:12Z
dc.date.available2014-06-20T14:13:12Z
dc.date.issued2012-12-01
dc.identifier.citationJeannine S. Strobl, David G. Goodwin, Beverly A. Rzigalinski, and David S. Lindsay (2012). "Dopamine Stimulates Propagation of Toxoplasma gondii Tachyzoites in Human Fibroblast and Primary Neonatal Rat Astrocyte Cell Cultures," Journal of Parasitology, Vol. 98, No. 6, pp. 1296-1299. doi: http://dx.doi.org/10.1645/GE-2760.1
dc.identifier.issn0022-3395
dc.identifier.urihttp://hdl.handle.net/10919/49037
dc.description.abstractToxoplasma gondii is an obligate intracellular parasite often found in the brain of humans. Research has shown a correlation between prevalence of antibody titers to T. gondii and psychological illness in humans. Recent studies indicate that individuals seropositive for T. gondii antibodies are more likely to develop psychotic disorders including schizophrenia, which is associated with changes in the dopamine neurotransmitter system. Dopamine in the brain may play a role in proliferation, chemoattraction, infection efficiency, or stage conversion of T. gondii. Because tachyzoites are the first developmental stage to reach the brain, the present study was conducted to determine the effects of dopamine on their development in vitro. In human fibroblast host cells, dopamine was added at either 100 nM or 250 nM to cell culture media, and the numbers of tachyzoites produced at 48 hr were determined and compared to vehicle-treated controls. An increase of tachyzoite numbers and increased destruction in cell monolayer were observed at both concentrations of dopamine. Dopamine used at 250 nM caused a significant (P < 0.05) increase in tachyzoites counts compared to controls. Dopamine antagonists (10 mu M) did not significantly alter dopamine-stimulated tachyzoite production in human fibroblasts. In primary neonatal rat astrocyte cell cultures, dopamine (200 mu M) significantly (P < 0.05) increased numbers of intracellular tachyzoites after 24 hr. The role that this increase plays in tachyzoite production under the stimulus of dopamine in the modulation of neural infection in humans awaits further studies.
dc.description.sponsorshipHarvey W. Peters Research Foundation
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_US
dc.publisherAmerican Society of Parasitology
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectHuman-skin fibroblastsen_US
dc.subjectBehavioral abnormalitiesen_US
dc.subjectMonoamine-oxidaseen_US
dc.subjectDecreased levelen_US
dc.subjectNovelty seekingen_US
dc.subjectInfected miceen_US
dc.subjectAciden_US
dc.subjectCytomegalovirusen_US
dc.subjectSchizophreniaen_US
dc.subjectDisordersen_US
dc.subjectParasitologyen_US
dc.titleDopamine Stimulates Propagation of Toxoplasma gondii Tachyzoites in Human Fibroblast and Primary Neonatal Rat Astrocyte Cell Cultures
dc.typeArticle - Refereed
dc.contributor.departmentElectrical and Computer Engineeringen_US
dc.contributor.departmentBiomedical Sciences and Pathobiologyen_US
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-2760.1
dc.date.accessed2014-06-16
dc.title.serialJournal of Parasitology
dc.identifier.doihttps://doi.org/10.1645/ge-2760.1
dc.type.dcmitypeText


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