Virginia Tech
    • Log in
    View Item 
    •   VTechWorks Home
    • Virginia-Maryland College of Veterinary Medicine (VMCVM)
    • Department of Biomedical Sciences and Pathobiology
    • Scholarly Works, Department of Biomedical Sciences and Pathobiology
    • View Item
    •   VTechWorks Home
    • Virginia-Maryland College of Veterinary Medicine (VMCVM)
    • Department of Biomedical Sciences and Pathobiology
    • Scholarly Works, Department of Biomedical Sciences and Pathobiology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Examination of extraintestinal tissue cysts of Isospora belli

    Thumbnail
    View/Open
    Main article (2.519Mb)
    Downloads: 960
    Date
    1997-08
    Author
    Lindsay, David S.
    Dubey, Jitender P.
    Toivio-Kinnucan, M. A.
    Michiels, J. F.
    Blagburn, B. L.
    Metadata
    Show full item record
    Abstract
    Relapse is common in immunocompetent and immunosuppressed humans infected with Isospora belli and is believed to be associated with the presence of extraintestinal stages. In the present study, we examined this important stage in an AIDS patient using histological, immunohistological, histochemical, and ultrastructural methods to better understand the development and structure of this stage and to develop better means of detecting infections. Antisera made in rabbits to Isospora suis, Toxoplasma gondii, Hammondia hammondi, Sarcocystis neurona, Neospora caninum, and Caryospora bigenetica were tested against I. belli tissue cysts in the avidin-biotin peroxidase complex (ABC) immunohistological test. Most antisera reacted positively in the ABC test at dilutions of 1:100 but not at dilutions of 1:250. Some antisera to N. caninum and H. hammondi reacted positively at dilutions of 1:1,000 in the ABC test. Most reactive antisera stained the tissue cyst wall and not the enclosed zoite. Eight histochemical tests were examined and most were nonreactive with I. belli zoites or tissue cysts. Transmission electron microscopy revealed that the tissue cyst wall was composed of granular material and was directly beneath the parasitophorous vacuole membrane. Zoites were in the center of the tissue cysts and were surrounded by fibrillar material that appeared to originate from the zoite surface. Tubulelike structures were present in the granular tissue cyst wall and in the fibrillar material that surrounded the zoite. Zoites contained a crystalloid body. New findings in the present study consisted of identifying what are probably early tissue cysts that lack a developed tissue cyst wall, demonstrating that more than 1 tissue cyst can occupy a host cell, describing the distribution of micronemes and the shedding of zoite membranes, and identifying tubular structures in the inner tissue cyst wall and inner compartment.
    URI
    http://hdl.handle.net/10919/49047
    Collections
    • Scholarly Works, Department of Biomedical Sciences and Pathobiology [464]

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us
     

     

    VTechWorks

    AboutPoliciesHelp

    Browse

    All of VTechWorksCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Log inRegister

    Statistics

    View Usage Statistics

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us