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dc.contributorVirginia Tech
dc.contributor.authorLindsay, D. S.
dc.contributor.authorMitchell, S. M.
dc.contributor.authorVianna, M. C.
dc.contributor.authorDubey, J. R.
dc.date.accessioned2014-06-20T14:13:22Z
dc.date.available2014-06-20T14:13:22Z
dc.date.issued2004-06
dc.identifier.citationDavid S. Lindsay, Sheila M. Mitchell, M. C. Vianna, and J. P. Dubey (2004). "Sarcocystis neurona (Protozoa : Apicomplexa): Description of oocysts, sporocysts, sporozoites, excystation, and early development," Journal of Parasitology, Vol. 90, No. 3, pp. 461-465. doi: http://dx.doi.org/10.1645/GE-230R
dc.identifier.issn0022-3395
dc.identifier.urihttp://hdl.handle.net/10919/49082
dc.description.abstractEquine protozoal myeloencephalitis is a major cause of neurological disease in horses from the Americas. Horses are considered accidental intermediate hosts. The structure of sporocysts of the causative agent, Sarcocystis neurona, has never been described. Sporocysts of S. neurona were obtained from the intestines of a laboratory-raised opossum fed skeletal muscles from a raccoon that had been fed sporocysts. Sporocysts were 11.3 by 8.2 mum and contained 4 sporozoites. The appearance of the sporocyst residuum was variable. The residuum of some sporocysts was composed of many dispersed granules, whereas some had granules mixed with larger globules. Excystation was by collapse of the sporocyst along plates. The sporocysts wall was composed of 3 layers: a thin electron-dense outer layer, a thin electron-lucent middle layer, and a thick electron-dense inner layer. The sporocyst wall was thickened at the junctions of the plates. Sporozoites were weakly motile and contained a centrally or posteriorly located nucleus. No retractile or crystalloid body was present, but lipidlike globules about I mum in diameter were usually present in the conoidal end of sporozoites. Sporozoites contained 2-4 electron-dense rhoptries and other organelles typical of coccidian zoites. Sporozoites entered host cells in culture and underwent schizogony within 3 days.
dc.description.sponsorshipUSDA/CSREES Animal Health and Disease grant VA-137180
dc.description.sponsorshipBayer HealthCare Animal Health
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_US
dc.publisherAmerican Society of Parasitology
dc.subjectopossums didelphis-virginiana
dc.subjectarmadillo dasypus-novemcinctus
dc.subjectinvitro
dc.subjectcultivation
dc.subjectexperimental induction
dc.subjectmyeloencephalitis epm
dc.subjectneurologic-disease
dc.subjectintermediate host
dc.subjectvascular phase
dc.subjectfalcatula
dc.subjectcruzi
dc.subjectparasitology
dc.titleSarcocystis neurona (Protozoa : Apicomplexa): Description of oocysts, sporocysts, sporozoites, excystation, and early development
dc.typeArticle - Refereed
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-230R
dc.date.accessed2014-06-16
dc.title.serialJournal of Parasitology
dc.identifier.doihttps://doi.org/10.1645/ge-230r
dc.type.dcmitypeText


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