Show simple item record

dc.contributor.authorRallos, Lynn Esther Espadaen_US
dc.date.accessioned2014-07-17T12:57:54Z
dc.date.available2014-07-17T12:57:54Z
dc.date.issued2013-01-21en_US
dc.identifier.othervt_gsexam:248en_US
dc.identifier.urihttp://hdl.handle.net/10919/49594
dc.description.abstractPractical resistance of Erysiphe necator to quinone outside inhibitors (QoIs) is now widespread, and resistance to demethylation inhibitors (DMIs) has also developed.  The goal of this research was to characterize fungicide resistance by elucidating resistance mechanisms and determining its stability.  QoI resistance persisted for several years in a field population after QoI application ended.  Resistant isolates were highly competitive in mixed populations in competition assays under laboratory conditions, indicating a lack of fitness cost.  In one competition trial under field conditions, resistance frequency declined, possibly due to spore migration and influx of background inoculum, but in a second trial, it did not decline.  Double resistance to QoI and DMI was detected and DMI application may have been partially responsible for maintaining QoI resistance in the field.  One isolate with QoI resistance but an undetectable level of the major QoI mutation was shown to be heteroplasmic -- resistant strains could be selected from this isolate.  

DMI resistance mechanisms in E. necator included the Y136F mutation in CYP51 and cyp51 over-expression.  The first mechanism was present in almost all isolates with substantial levels of resistance, and cyp51 expression level was correlated with resistance level.  Three cyp51 genotypes were detected.  Wildtype isolates with the TAT genotype were sensitive to DMIs, while isolates with increased resistance had either a TTT or TWT genotype; TWT indicated the presence of both wildtype and mutant alleles.  Cyp51 was expressed 1.4 to 19 times more in mutants than in wildtype.  It is not known whether the significant differences in cyp51 expression level among isolates and among genotype groups are due to gene copy number variation.  DMI resistance was found to decline after years of subculturing, and the decline appeared to occur after a few culture transfers of field samples on fungicide-free host leaves.  The observed decline, together with the finding that isolates could be "trained" to increase resistance, and may be slightly induced in cyp51 expression when successively challenged to grow in increasing fungicide concentration, indicate instability of DMI resistance.
en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsThis Item is protected by copyright and/or related rights. Some uses of this Item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en_US
dc.subjectErysphe necatoren_US
dc.subjectfungicide resistanceen_US
dc.subjectQoIen_US
dc.subjectDMIen_US
dc.subjectcytochrome ben_US
dc.subjectCYP51en_US
dc.subjectresistance mechanismsen_US
dc.titleCharacterizing resistance of Erysiphe necator to fungicides belonging to the quinone outside inhibitors and demethylation inhibitorsen_US
dc.typeDissertationen_US
dc.contributor.departmentPlant Pathology, Physiology, and Weed Scienceen_US
dc.description.degreePh. D.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplinePlant Pathology, Physiology, and Weed Scienceen_US
dc.contributor.committeechairBaudoin, Antonius B.en_US
dc.contributor.committeememberSchmale, David Garner Burtonen_US
dc.contributor.committeememberHong, Chuanxueen_US
dc.contributor.committeememberVinatzer, Boris A.en_US
dc.contributor.committeememberNita, Mizuhoen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record