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dc.contributor.authorZhang, Husenen_US
dc.contributor.authorWang, Haifengen_US
dc.contributor.authorShepherd, Meganen_US
dc.contributor.authorWen, Keen_US
dc.contributor.authorLi, Guohuaen_US
dc.contributor.authorYang, Xingdongen_US
dc.contributor.authorKocher, Jacoben_US
dc.contributor.authorGiri-Rachman, Ernawatien_US
dc.contributor.authorDickerman, Allanen_US
dc.contributor.authorSettlage, Roberten_US
dc.contributor.authorYuan, Lijuanen_US
dc.identifier.citationGut Pathogens. 2014 Sep 09;6(1):39en_US
dc.description.abstractWe generated a neonatal pig model with human infant gut microbiota (HGM) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. All the HGM-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. The gut microbiota of vaccinated pigs were investigated for effects of Lactobacillus rhamnosus GG (LGG) supplement and homotypic virulent human rotavirus (HRV) challenge. High-throughput sequencing of V4 region of 16S rRNA genes demonstrated that HGM-transplanted pigs carried microbiota similar to that of the C-section delivered baby. Firmicutes and Proteobacteria represented over 98% of total bacteria in the human donor and the recipient pigs. HRV challenge caused a phylum-level shift from Firmicutes to Proteobacteria. LGG supplement prevented the changes in microbial communities caused by HRV challenge. In particular, members of Enterococcus in LGG-supplemented pigs were kept at the baseline level, while they were enriched in HRV challenged pigs. Taken together, our results suggested that HGM pigs are valuable for testing the microbiota’s response to probiotic interventions for treating infantile HRV infection.en_US
dc.rightsCreative Commons Attribution 4.0 International*
dc.titleProbiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigsen_US
dc.typeArticle - Refereed
dc.description.versionPeer Reviewed
dc.rights.holderHusen Zhang et al.; licensee BioMed Central Ltd.en_US
dc.title.serialGut Pathogens

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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International