Show simple item record

dc.contributor.authorTae, Hongseoken_US
dc.contributor.authorKarunasena, Enushaen_US
dc.contributor.authorBavarva, Jasmin Hen_US
dc.contributor.authorGarner, Harold R.en_US
dc.identifier.citationBioData Mining. 2014 Nov 07;7(1):25en_US
dc.description.abstractBackground Many bacterial genome sequences completed using the Sanger method may contain assembly errors due in-part to low sequence coverage driven by cost. Findings To illustrate the need for re-sequencing of pre-nextgen genomes and to validate sequenced genomes, we conducted a series of experiments, using high coverage sequencing data generated by a Illumina Miseq sequencer to sequence genomic DNAs of Bacteroides fragilis NCTC 9343, Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150, Vibrio cholerae O1 biovar El Tor str. N16961, Bacillus halodurans C-125 and Caulobacter crescentus CB15, which had previously been sequenced by the Sanger method during the early 2000’s. Conclusions This study revealed a number of discrepancies between the published assemblies and sequence read alignments for all five bacterial species, suggesting that the continued use of these error-containing genomes and their genetic information may contribute to false conclusions and/or incorrect future discoveries when they are used.en_US
dc.rightsCreative Commons Attribution 4.0 International*
dc.titleUpdating microbial genomic sequences: improving accuracy & innovationen_US
dc.typeArticle - Refereed
dc.description.versionPeer Reviewed
dc.rights.holderHongseok Tae et al.; licensee BioMed Central Ltd.en_US
dc.title.serialBioData Mining

Files in this item


This item appears in the following Collection(s)

Show simple item record

Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International