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dc.contributorVirginia Tech. Department of Biological Sciences
dc.contributor.authorLazar, Iulia M.
dc.contributor.authorKabulski, Jarod L.
dc.date.accessioned2015-04-20T21:04:11Z
dc.date.available2015-04-20T21:04:11Z
dc.date.issued2013-03-20
dc.identifier.citationLazar, I. M., & Kabulski, J. L. (2013). Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection. Lab on a Chip, 13(11), 2055-2065. doi: 10.1039/C3LC50190F
dc.identifier.issn1473-0197
dc.identifier.urihttp://hdl.handle.net/10919/51708
dc.description.abstractA microfluidic device that enables on-chip matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) detection for liquid chromatography (LC) separations is described. The device comprises an array of functional elements to carry out LC separations, integrates a novel microchip-MS interface to facilitate the orthogonal transposition of the microfluidic LC channel into an array of reservoirs, and enables sensitive MALDI-MS detection directly from the chip. Essentially, the device provides a snapshot MALDI-MS map of the content of the separation channel present on the chip. The detection of proteins with biomarker potential from MCF10A breast epithelial cell extracts, and detection limits in the low fmol range, are demonstrated. In addition, the design of the novel LC-MALDI-MS chip entices the promotion of a new concept for performing sample separations within the limited time-frame that accompanies the dead-volume of a separation channel.
dc.description.sponsorshipNational Institutes of Health. National Cancer Institute - Award Number R21CA126669-01A1
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/vnd.ms-excel
dc.format.mimetypeimage/jpeg
dc.language.isoen_US
dc.publisherThe Royal Society of Chemistry
dc.subjectMass spectrometry
dc.subjectLiquid chromatography
dc.subjectMatrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)
dc.titleMicrofluidic LC Device with Orthogonal Sample Extraction for On-Chip MALDI-MS Detection
dc.typeArticle - Refereed
dc.typeDataset
dc.typeImage
dc.description.notesSupplementary information is included in separate files
dc.identifier.urlhttp://pubs.rsc.org/en/content/articlelanding/2013/lc/c3lc50190f
dc.date.accessed2015-04-17
dc.title.serialLab on a Chip
dc.identifier.doihttps://doi.org/10.1039/C3LC50190F
dc.type.dcmitypeText
dc.type.dcmitypeDataset
dc.type.dcmitypeImage


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