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dc.contributorVirginia Tech. Department of Biological Systems Engineeringen
dc.contributor.authorRidgley, Devin M.en
dc.contributor.authorFreedman, Benjamin G.en
dc.contributor.authorLee, Parker W.en
dc.contributor.authorBarone, Justin R.en
dc.identifier.citationRidgley, D. M., Freedman, B. G., Lee, P. W., & Barone, J. R. (2014). Genetically encoded self-assembly of large amyloid fibers. Biomaterials Science, 2(4), 560-566. doi: 10.1039/C3BM60223Ken
dc.description.abstract“Functional” amyloids are found throughout nature as robust materials. We have discovered that “template” and “adder” proteins cooperatively self-assemble into micrometer-sized amyloid fibers with a controllable, hierarchical structure. Here, Escherichia coli is genetically engineered to express a template protein, Gd20, that can initiate self-assembly of large amyloid fibrils and fibers. Through atomic force microscopy (AFM) we found that expression of Gd20 produces large amyloid fibrils of 490 nm diameter and 2–15 _m length. Addition of an extracellular adder protein, myoglobin, continues self-assembly to form amyloid tapes with widths of [similar]7.5 _m, heights of [similar]400 nm, and lengths exceeding 100 _m. Without myoglobin the amyloid fibrils are metastable over time. When myoglobin is present, the amyloid fiber continues self-assembling to a width of [similar]18 _m and height of [similar]1 _m. Experimental results demonstrate that large amyloid fibers with a tailored stiffness and morphology can be engineered at the DNA level, spanning four orders of magnitude.en
dc.description.sponsorshipNational Science Foundation (U.S.) - NSF-CMMI-0856262en
dc.description.sponsorshipVirginia Tech. College of Agriculture and Life Sciences. Biodesign and Bioprocessing Research Centeren
dc.description.sponsorshipU.S. Department of Agricultureen
dc.publisherThe Royal Society of Chemistryen
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.subjectFourier-transform infrared (FT-IR) spectroscopyen
dc.subjectGd20 plasmid expressionen
dc.titleGenetically Encoded Self-Assembly of Large Amyloid Fibersen
dc.typeArticle - Refereeden
dc.contributor.departmentBiological Systems Engineeringen
dc.description.notesSupplementary information is included in a separate fileen
dc.title.serialBiomaterials Scienceen

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