Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

Files
2014_Sun_Lu_et_al.pdf (2.12 MB)
Downloads: 321

2014_Sun_Lu_et_al_SUPPLEMENT.pdf (213.27 KB)
Downloads: 60
TR Number
Date
2014-08-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
The Royal Society of Chemistry
Abstract

Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ~100 cells with high spatiotemporal resolution.

Description
Keywords
Fluorescence resonance energy transfer, Biosensors, Genetic encoding
Citation
Sun, C., Ouyang, M., Cao, Z., Ma, S., Alqublan, H., Sriranganathan, N., Wang, Y., Lu, C. (2014). Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding. Chemical Communications, 50(78), 11536-11539. doi: 10.1039/C4CC04730C
Persistent link
http://hdl.handle.net/10919/51723
Collections
Scholarly Works, School of Biomedical Engineering and Sciences