Show simple item record

dc.contributorVirginia Tech. School of Biomedical Engineering and Sciencesen
dc.contributorVirginia Tech. Institute for Critical Technology and Applied Scienceen
dc.contributorVirginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiologyen
dc.contributorVirginia Tech. Mechanical Engineering Departmenten
dc.contributor.authorSharma, Pujaen
dc.contributor.authorSheets, Kevinen
dc.contributor.authorElankumaran, Subbiahen
dc.contributor.authorNain, Amrinder S.en
dc.date.accessioned2015-04-20T22:22:12Zen
dc.date.available2015-04-20T22:22:12Zen
dc.date.issued2013-06-03en
dc.identifier.citationSharma, P., Sheets, K., Elankumaran, S., & Nain, A. S. (2013). The mechanistic influence of aligned nanofibers on cell shape, migration and blebbing dynamics of glioma cells. Integrative Biology, 5(8), 1036-1044. doi: 10.1039/C3IB40073Een
dc.identifier.issn1757-9694en
dc.identifier.urihttp://hdl.handle.net/10919/51728en
dc.description.abstractInvestigating the mechanistic influence of the tumor microenvironment on cancer cell migration and membrane blebbing is crucial in the understanding and eventual arrest of cancer metastasis. In this study, we investigate the effect of suspended and aligned nanofibers on the glioma cytoskeleton, cell shape, migration and plasma membrane blebbing dynamics using a non-electrospinning fiber-manufacturing platform. Cells attached in repeatable shapes of spindle on single fibers, rectangular on two parallel fibers and polygonal on intersecting fibers. Structural stiffness (N m_1) of aligned and suspended nanofibers (average diameter: 400 nm, length: 4, 6, and 10 mm) was found to significantly alter the migration speed with higher migration on lower stiffness fibers. For cells attached to fibers and exhibiting blebbing, an increase in cellular spread area resulted in both reduced bleb count and bleb size with an overall increase in cell migration speed. Blebs no longer appeared past a critical cellular spread area of approximately 1400 _m2. Our results highlighting the influence of the mechanistic environment on the invasion dynamics of glioma cells add to the understanding of how biophysical components influence glioma cell migration and blebbing dynamics.en
dc.description.sponsorshipJeffress Memorial Trusten
dc.description.sponsorshipAndrea and Bill Waideen
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeimage/jpegen
dc.format.mimetypetext/plainen
dc.language.isoen_USen
dc.publisherThe Royal Society of Chemistryen
dc.relation.ispartofseriesOpen access articles from Integrative Biologyen
dc.relation.urihttp://pubs.rsc.org/en/journals/articlecollectionlanding?sercode=ib&themeid=ab0f94be-eff2-4286-b96e-367458372850en
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectNanofibersen
dc.subjectTumorsen
dc.subjectGlioblastoma multiformeen
dc.subjectCancer cell migrationen
dc.titleThe Mechanistic Influence of Aligned Nanofiber Networks on Cell Shape, Migration and Blebbing Dynamics of Glioma Cellsen
dc.typeArticle - Refereeden
dc.typeImageen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.description.notesSupplementary information is included in separate files. A supplementary video can be accessed at the following URL - http://www.rsc.org/suppdata/ib/c3/c3ib40073e/c3ib40073e.wmven
dc.identifier.urlhttp://pubs.rsc.org/en/content/articlelanding/2013/ib/c3ib40073een
dc.date.accessed2015-04-17en
dc.title.serialIntegrative Biologyen
dc.identifier.doihttps://doi.org/10.1039/C3IB40073Een
dc.type.dcmitypeTexten
dc.type.dcmitypeImageen
dc.type.dcmitypeStillImageen


Files in this item

Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Creative Commons Attribution-NonCommercial 3.0 Unported
License: Creative Commons Attribution-NonCommercial 3.0 Unported