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dc.contributorVirginia Tech. Department of Plant Pathology, Physiology, and Weed Science
dc.contributorUniversity of Pretoria. Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI)
dc.contributorUniversity of Pretoria. Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI)
dc.contributor.authorMyburg, Henrietta
dc.contributor.authorGryzenhout, Marieka
dc.contributor.authorWingfield, Brenda D.
dc.contributor.authorStipes, R. Jay
dc.contributor.authorWingfield, Michael J.
dc.date.accessioned2015-04-23T19:36:27Z
dc.date.available2015-04-23T19:36:27Z
dc.date.issued2004
dc.identifier.citationMyburg, H., Gryzenhout, M., Wingfield, B. D., Stipes, R. J. & Wingfield, M. J. (2004). Phylogenetic relationships of Cryphonectria and Endothia species, based on DNA sequence data and morphology. Mycologia, 96(5), 990-1001. doi: 10.2307/3762083
dc.identifier.issn0027-5514
dc.identifier.urihttp://hdl.handle.net/10919/51786
dc.description.abstractThe fungal genera Endothia and Cryphonectria include some of the most important pathogens of forest trees. Despite available new technology, no comprehensive comparative study based on DNA sequence data and morphology has been done on the available isolates representing these two genera. The main objectives of this study were to assess the phylogenetic relationships among species of Cryphonectria and Endothia, for which cultures are available, and to establish a taxonomic framework based on DNA sequence and morphological data, which will aid future studies and identification of species in these and related genera. Comparisons were based on sequence variation found in the ITS region of the ribosomal RNA operon and two regions of the P-tubulin gene. In addition, the morphology of these species was examined. The phylogenetic data indicated that Endothia and Cryphonectria reside in two distinct phylogenetic clades. Cryphonectria parasitica, C. macrospora, C. nitschkei, C. eucalypti and C. radicalis represented the Cryphonectria clade. Endothia gyrosa and E. singularis were included in the Endothia clade. An isolate representing E. viridistroma grouped outside the Endothia clade and separately from other groups. Other clades outside the one encompassing Cryphonectria were those represented by the C. cubensis isolates and fungi isolated from Elaeocarpus dentalus originating from New Zealand. These clades could be distinguished from Endothia and Cryphonectria, based on anamorph morphology, stromatal structure and ascospore septation. Cryphonectria and Endothia, therefore, appear to be paraphyletic and taxonomic relationships for these fungi need to be revised.
dc.description.sponsorshipNational Research Foundation (South Africa)
dc.description.sponsorshipUniversity of Pretoria. Forestry and Agricultural Biotechnology Institute. Tree Pathology Co-operative Programme (TPCP)
dc.description.sponsorshipTHRIP
dc.format.extent12 pages
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherMycological Society of America
dc.subject_-tubulin genes
dc.subjectCryphonectria
dc.subjectDiaporthales
dc.subjectEndothia
dc.subjectPhylogeny
dc.subjectRibosomal DNA
dc.titlePhylogenetic relationships of Cryphonectria and Endothia species, based on DNA sequence data and morphology
dc.typeArticle - Refereed
dc.identifier.urlhttp://www.mycologia.org/content/96/5/990.abstract?sid=fd667f87-8cc4-4e30-a0c8-16b0e404253f
dc.date.accessed2015-04-21
dc.title.serialMycologia
dc.identifier.doihttps://doi.org/10.2307/3762083
dc.type.dcmitypeText


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