Show simple item record

dc.contributor.authorRychkov, Georgyen_US
dc.contributor.authorNazarov, Igoren_US
dc.contributor.authorIlatovskiy, Andreyen_US
dc.contributor.authorShvetsov, Alexeyen_US
dc.contributor.authorKonev, Alexanderen_US
dc.contributor.authorIsaev-Ivanov, Vladimiren_US
dc.contributor.authorOnufriev, Alexey V.en_US
dc.date.accessioned2015-05-22T01:11:45Z
dc.date.available2015-05-22T01:11:45Z
dc.date.issued2015-05-21
dc.identifier.urihttp://hdl.handle.net/10919/52506
dc.description.abstractEvidence is now overwhelming that partially assembled states of the nucleosome are as important as the canonical structure for the understanding of how DNA accessibility is regulated in cells. We use a combination of atomistic simulation and Atomic Force Microscopy (AFM) to propose models of key partially assembled structures of the nucleosome at atomic detail: the heaxasome ((H3·H4)2·(H2A·H2B)), the tetrasome ((H3·H4)2), and the disome (H3·H4). Despite large-scale fluctuations of the “free” DNA not in direct contact with the histones in these structures, the histone-DNA contacts are stable, establishing the basis for interpretation of the role of these structures in providing variable degree of DNA accessibility within the chromatin. We show how the atomistically detailed partially assembled nucleosome structures can be used to interpret experimental observations, both in-vitro and in-vivo. Specifically, interpretation of FRET, AFM, (and SAXS) experiments under conditions where partially assembled nucleosome states are expected is greatly facilitated by the availability of atomic- resolution structural ensembles of these states. We also suggest an alternative interpretation of a recent genome-wide study that investigated types of DNA protection in classical “active” chromatin, lending support for the transcription speed-up scenario that involves partially assembled sub-nucleosome structures.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoenen_US
dc.publisherDepartment of Computer Science, Virginia Polytechnic Institute & State Universityen_US
dc.relation.ispartofComputer Science Technical Reportsen_US
dc.rightsAttribution-NonCommercial 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/us/*
dc.subjectComputational biologyen_US
dc.subjectBioinformaticsen_US
dc.titlePartially assembled nucleosome structures at atomic detailen_US
dc.typeTechnical reporten_US
dc.identifier.trnumberTR-15-03en_US
dc.type.dcmitypeTexten_US


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial 3.0 United States
License: Attribution-NonCommercial 3.0 United States