Now showing items 1-4 of 4
Diffusion-based Microfluidic PCR for "One-pot" Analysis of Cells
(The Royal Society of Chemistry, 2014-05-28)
Genetic analysis starting with cell samples often requires multi-step processing including cell lysis, DNA isolation/purification, and polymerase chain reaction (PCR) based assays. When conducted on a microfluidic platform, ...
Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding
(The Royal Society of Chemistry, 2014-08-08)
Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using ...
Electroporation-based delivery of cell-penetrating peptide conjugates of peptide nucleic acids for antisense inhibition of intracellular bacteria
(The Royal Society of Chemistry, 2014-08-14)
Cell penetrating peptides (CPPs) have been used for a myriad of cellular delivery applications and were recently explored for delivery of antisense agents such as peptide nucleic acids (PNAs) for bacterial inhibition. ...
Focusing of mammalian cells under an ultrahigh pH gradient created by unidirectional electropulsation in a confined microchamber
(The Royal Society of Chemistry, 2014-06-09)
The transport and manipulation of cells in microfluidic structures are often critically required in cellular analysis. Cells typically make consistent movement in a dc electric field in a single direction, due to their ...