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dc.contributor.authorSrinivasaraghavan, Vaishnavien_US
dc.date.accessioned2015-11-05T09:00:37Z
dc.date.available2015-11-05T09:00:37Z
dc.date.issued2015-11-04en_US
dc.identifier.othervt_gsexam:6390en_US
dc.identifier.urihttp://hdl.handle.net/10919/63921
dc.description.abstractBioimpedance presents a versatile, label-free means of monitoring biological cells and their responses to physical, chemical and biological stimuli. Breast cancer is the second most common type of cancer among women in the United States. Although significant progress has been made in diagnosis and treatment of this disease, there is a need for robust, easy-to-use technologies that can be used for the identification and discrimination of critical subtypes of breast cancer in biopsies obtained from patients. This dissertation makes contributions in three major areas towards addressing the goal. First, we developed miniaturized bioimpedance sensors using MEMS and microfluidics technology that have the requisite traits for clinical use including reliability, ease-of-use, low-cost and disposability. Here, we designed and fabricated two types of bioimpedance sensors. One was based on electric cell-substrate impedance sensing (ECIS) to monitor cell adhesion based events and the other was a microfluidic device with integrated microelectrodes to examine the biophysical properties of single cells. Second, we examined a panel of triple negative breast cancer (TNBC) cell lines and a hormone therapy resistant model of breast cancer in order to improve our understanding of the bioimpedance spectra of breast cancer subtypes. Third, we explored strategies to improve the sensitivity of the microelectrodes to bioimpedance measurements from breast cancer cells. We investigated nano-scale coatings on the surface of the electrode and geometrical variations in a branched electrode design to accomplish this. This work demonstrates the promise of bioimpedance technologies in monitoring diseased cells and their responses to pharmaceutical agents, and motivates further research in customization of this technique for use in personalized medicine.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsThis Item is protected by copyright and/or related rights. Some uses of this Item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en_US
dc.subjectMicroelectromechanical systems (MEMS)en_US
dc.subjectBioimpedanceen_US
dc.subjectMicroelectrode Arrays (MEA)en_US
dc.subjectElectrical Cell-substrate Impedance Sensing (ECIS)en_US
dc.subjectBreast Canceren_US
dc.subjectTriple Negative Breast Cancer (TNBC)en_US
dc.subjectNano-coatingsen_US
dc.subjectSingle cell analysisen_US
dc.subjectMicrofluidicsen_US
dc.titleBioimpedance spectroscopy of breast cancer cells: A microsystems approachen_US
dc.typeDissertationen_US
dc.contributor.departmentElectrical and Computer Engineeringen_US
dc.description.degreePh. D.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineElectrical Engineeringen_US
dc.contributor.committeechairAgah, Masouden_US
dc.contributor.committeememberZhu, Yizhengen_US
dc.contributor.committeememberLester, Luke F.en_US
dc.contributor.committeememberStrobl, Jeannine Susanen_US
dc.contributor.committeememberKelly, Deborah F.en_US


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