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dc.contributorVirginia Techen_US
dc.contributor.authorIakhiaeva, Elenaen_US
dc.contributor.authorMcNulty, Stevenen_US
dc.contributor.authorBrown-Elliott, Barbara A.en_US
dc.contributor.authorFalkinham, Joseph O. IIIen_US
dc.contributor.authorWilliams, Myra D.en_US
dc.contributor.authorVasireddy, Ravikiranen_US
dc.contributor.authorWilson, Rebecca W.en_US
dc.contributor.authorTurenne, Christineen_US
dc.contributor.authorWallace, Richard J. Jr.en_US
dc.date.accessioned2015-11-29T04:13:27Z
dc.date.available2015-11-29T04:13:27Z
dc.date.issued2012-11-21en_US
dc.identifier.citationIakhiaeva, Elena et al. (2013). Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat (MIRU-VNTR) Genotyping of Mycobacterium intracellulare for Strain Comparison with Establishment of a PCR-Based Database. Journal of Clinical Microbiology, 51(2), 409-416. doi:10.1128/jcm.02443-12en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10919/64224
dc.description.abstractStrain comparison is important to population genetics and to evaluate relapses in patients with Mycobacterium avium complex (MAC) lung disease, but the "gold standard" of pulsed-field gel electrophoresis (PFGE) is time-consuming and complex. We used variable-number tandem repeats (VNTR) for fingerprinting of respiratory isolates of M. intracellulare from patients with underlying bronchiectasis, to establish a nonsequence-based database for population analysis. Different genotypes identified by PFGE underwent species identification using a 16S rRNA gene multiplex PCR. Genotypes of M. intracellulare were confirmed by internal transcribed spacer 1 (ITS1) sequencing and characterized using seven VNTR primers. The pattern of VNTR amplicon sizes and repeat number defined each specific VNTR type. Forty-two VNTR types were identified among 84 genotypes. PFGE revealed most isolates with the same VNTR type to be clonal or exhibit similar grouping of bands. Repetitive sequence-based PCR (rep-PCR) showed minimal pattern diversity between VNTR types compared to PFGE. Fingerprinting of relapse isolates from 31 treated patients using VNTR combined with 16S multiplex PCR unambiguously and reliably distinguished different genotypes from the same patient, with results comparable to those of PFGE. VNTR for strain comparison is easier and faster than PFGE, is as accurate as PFGE, and does not require sequencing. Starting with a collection of 167 M. intracellulare isolates, VNTR distinguished M. intracellulare into 42 clonal groups. Comparison of isolates from different geographic areas, habitats, and clinical settings is now possible.en_US
dc.description.sponsorshipAmon G. Carter Foundationen_US
dc.description.sponsorshipNTM-IR private foundationen_US
dc.description.sponsorshipThe University of Texas Health Science Center at Tyleren_US
dc.description.sponsorshipVirginia Tech. Department of Biological Sciencesen_US
dc.description.sponsorshipSaskatchewan Disease Control Laboratoryen_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titleMycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat (MIRU-VNTR) Genotyping of Mycobacterium intracellulare for Strain Comparison with Establishment of a PCR-Based Databaseen_US
dc.typeArticle - Refereeden_US
dc.typeDataseten_US
dc.rights.holderAmerican Society for Microbiologyen
dc.contributor.departmentBiological Sciencesen_US
dc.identifier.urlhttp://jcm.asm.org/content/51/2/409en_US
dc.date.accessed2015-11-28en_US
dc.title.serialJournal of Clinical Microbiologyen_US
dc.identifier.doihttps://doi.org/10.1128/jcm.02443-12
dc.type.dcmitypeTexten_US
dc.type.dcmitypeDataseten_US


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