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dc.contributor.authorHochendoner, Philip Louisen_US
dc.date.accessioned2015-12-26T09:04:53Z
dc.date.available2015-12-26T09:04:53Z
dc.date.issued2015-12-12en_US
dc.identifier.othervt_gsexam:6884en_US
dc.identifier.urihttp://hdl.handle.net/10919/64373
dc.description.abstractThe bulk of this thesis considers how biological rhythms (oscillators) can be made to synchronize their rhythms by virtue of coupling to an external signal. Such externally controlled synchronization, known as entrainment, is explored using a synthetic biology approach in E.~coli, where I have used rationally designed gene circuits as an experimental model. Two novel modes of entrainment are explored: entrainment by competition between components for degradation, and entrainment by a noisy (aperiodic) stimulus. Both of these modes of entrainment can be shown to strongly synchronize ensembles of synthetic gene oscillators, and thus, these modes of entrainment may be important to understand the appearance of synchrony in natural systems. In addition to the study of entrainment, this thesis contains a general background of relevant material, contributions to the biophysics of multisite proteases, and updated protocols for experimental procedures in microfluidics and microscopy.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsThis Item is protected by copyright and/or related rights. Some uses of this Item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en_US
dc.subjectBiophysicsen_US
dc.subjectSynthetic Biologyen_US
dc.subjectEntrainmenten_US
dc.subjectQueueingen_US
dc.titleEntrainment of Bacterial Synthetic Oscillators using Proteolytic Queueing and Aperiodic Signalingen_US
dc.typeDissertationen_US
dc.contributor.departmentPhysicsen_US
dc.description.degreePh. D.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplinePhysicsen_US
dc.contributor.committeechairMather, Williamen_US
dc.contributor.committeememberPleimling, Michel Jeanen_US
dc.contributor.committeememberRobinson, Hansen_US
dc.contributor.committeememberCheng, Shengfengen_US


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