Overfertilization can detrimentally affect both the environment and farmer income and often results from the fertilization of unresponsive fields. The current methodology for detecting unresponsive fields via determination of amino sugar nitrogen in soil hydrolysates is both complicated and time-consuming. In this study, a novel amino sugar nitrogen soil test is developed and compared to testing using hydrolysates using soil samples from twenty-five sites. Air-dried soil was heated and treated with sodium hydroxide to release ammonia, which was then titrated to determine the total amino sugar nitrogen in the original sample. Results were highly comparable to the existing hydrolysate methodology and confirm that this process will be effective in determining responsiveness to nitrogen fertilization and preventing the environmental pollution resulting from over-application.