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dc.contributor.authorRay, Parthaen_US
dc.contributor.authorKnowlton, Katharine F.en_US
dc.contributor.authorShang, Chaoen_US
dc.contributor.authorXia, Kangen_US
dc.date.accessioned2016-12-06T16:55:20Z
dc.date.available2016-12-06T16:55:20Z
dc.date.issued2014-11-06en_US
dc.identifier.citationdc.identifier.citation Ray P, Knowlton KF, Shang C, Xia K (2014) Development and Validation of a UPLC-MS/MS Method to Monitor Cephapirin Excretion in Dairy Cows following Intramammary Infusion. PLoS ONE 9(11): e112343. doi:10.1371/journal.pone.0112343
dc.identifier.issn1932-6203en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10919/73568
dc.description.abstractCephapirin, a cephalosporin antibiotic, is used by the majority of dairy farms in the US. Fecal and urinary excretion of cephapirin could introduce this compound into the environment when manure is land applied as fertilizer, and may cause development of bacterial resistance to antibiotics critical for human health. The environmental loading of cephapirin by the livestock industry remains un-assessed, largely due to a lack of appropriate analytical methods. Therefore, this study aimed to develop and validate a cephapirin quantification method to capture the temporal pattern of cephapirin excretion in dairy cows following intramammary infusion. The method includes an extraction with phosphate buffer and methanol, solid-phase extraction (SPE) clean-up, and quantification using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The LOQ values of the developed method were 4.02 µg kg−1 and 0.96 µg L−1 for feces and urine, respectively. This robust method recovered >60% and >80% cephapirin from spiked blank fecal and urine samples, respectively, with acceptable intra- and inter-day variation (<10%). Using this method, we detected trace amounts (µg kg−1) of cephapirin in dairy cow feces, and cephapirin in urine was detected at very high concentrations (133 to 480 µg L−1). Cephapirin was primarily excreted via urine and its urinary excretion was influenced by day (P = 0.03). Peak excretion (2.69 mg) was on day 1 following intramammary infusion and decreased sharply thereafter (0.19, 0.19, 0.08, and 0.17 mg on day 2, 3, 4, and 5, respectively) reflecting a quadratic pattern of excretion (Quadratic: P = 0.03). The described method for quantification of cephapirin in bovine feces and urine is sensitive, accurate, and robust and allowed to monitor the pattern of cephapirin excretion in dairy cows. This data will help develop manure segregation and treatment methods to minimize the risk of antibiotic loading to the environment from dairy farms.
dc.description.sponsorshipVirginia Tech. College of Agriculture and Life Sciences
dc.description.sponsorshipVirginia Tech. Open Access Subvention Fund
dc.description.sponsorshipVirginia Cooperative Extension
dc.description.sponsorshipVirginia Agricultural Experiment Station
dc.format.extent? - ? (12) page(s)en_US
dc.format.extent12 p.
dc.format.mimetypeapplication/pdfen_US
dc.languageEnglishen_US
dc.languageen_US
dc.publisherPLOSen_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000344402600133&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMultidisciplinary Sciencesen_US
dc.subjectScience & Technology - Other Topicsen_US
dc.subjectTANDEM MASS-SPECTROMETRYen_US
dc.subjectLIQUID-CHROMATOGRAPHYen_US
dc.subjectANTIBIOTIC-RESISTANCEen_US
dc.subjectTISSUE DISTRIBUTIONen_US
dc.subjectVETERINARY ANTIBIOTICSen_US
dc.subjectSTAPHYLOCOCCUS-AUREUSen_US
dc.subjectCEFTIOFUR SODIUMen_US
dc.subjectBOVINE MASTITISen_US
dc.subjectMANURE STORAGEen_US
dc.subjectDRUG RESIDUESen_US
dc.titleDevelopment and Validation of a UPLC-MS/MS Method to Monitor Cephapirin Excretion in Dairy Cows following Intramammary Infusionen_US
dc.typeArticle - Refereeden_US
dc.description.versionPublished (Publication status)en_US
dc.title.serialPLOS ONEen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0112343
dc.identifier.volume9en_US
dc.identifier.issue11en_US
dc.type.dcmitypeTexten_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Crop & Soil Environmental Science
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Dairy Science
pubs.organisational-group/Virginia Tech/All T&R Faculty


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Creative Commons Attribution 4.0 International (CC BY 4.0)
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