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dc.contributor.authorSerazin, Aandrew C.en_US
dc.contributor.authorDana, Ali N.en_US
dc.contributor.authorHillenmeyer, Maureen E.en_US
dc.contributor.authorLobo, Neil F.en_US
dc.contributor.authorCoulibaly, Mamadou B.en_US
dc.contributor.authorWillard, Michael B.en_US
dc.contributor.authorHarker, Brent W.en_US
dc.contributor.authorSharakhov, Igor V.en_US
dc.contributor.authorCollins, Frank H.en_US
dc.contributor.authorRibeiro, Jose M. C.en_US
dc.contributor.authorBesansky, Nora J.en_US
dc.coverage.spatialUnited Statesen_US
dc.description.abstractBACKGROUND: Anopheles funestus is a principal vector of malaria across much of tropical Africa and is considered one of the most efficient of its kind, yet studies of this species have lagged behind those of its broadly sympatric congener, An. gambiae. In aid of future genomic sequencing of An. funestus, we explored the whole body transcriptome, derived from mixed stage progeny of wild-caught females from Mali, West Africa. PRINCIPAL FINDINGS: Here we report the functional annotation and comparative genomics of 2,005 expressed sequence tags (ESTs) from An. funestus, which were assembled with a previous EST set from adult female salivary glands from the same mosquito. The assembled ESTs provided for a nonredundant catalog of 1,035 transcripts excluding mitochondrial sequences. CONCLUSIONS/SIGNIFICANCE: Comparison of the An. funestus and An. gambiae transcriptomes using computational and macroarray approaches revealed a high degree of sequence identity despite an estimated 20-80 MY divergence time between lineages. A phylogenetically broader comparative genomic analysis indicated that the most rapidly evolving proteins--those involved in immunity, hematophagy, formation of extracellular structures, and hypothetical conserved proteins--are those that probably play important roles in how mosquitoes adapt to their nutritional and external environments, and therefore could be of greatest interest in disease control.en_US
dc.format.extente7976 - ? page(s)en_US
dc.rightsCC0 1.0 Universal (CC0 1.0) Public Domain Dedication*
dc.subjectComputational Biologyen_US
dc.subjectDNA, Complementaryen_US
dc.subjectExpressed Sequence Tagsen_US
dc.subjectGene Expression Profilingen_US
dc.subjectGene Libraryen_US
dc.subjectSalivary Glandsen_US
dc.subjectTranscription, Geneticen_US
dc.titleComparative analysis of the global transcriptome of Anopheles funestus from Mali, West Africa.en_US
dc.typeArticle - Refereed
dc.description.versionPublished online (Publication status)en_US
dc.title.serialPLoS Oneen_US
dc.type.otherComparative Studyen_US
dc.type.otherResearch Support, N.I.H., Extramuralen_US
dc.type.otherResearch Support, N.I.H., Intramuralen_US
dc.type.otherResearch Support, Non-U.S. Gov'ten_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomology
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences

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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
License: CC0 1.0 Universal (CC0 1.0) Public Domain Dedication