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dc.contributor.authorPeery, Ashleyen_US
dc.contributor.authorSharakhova, Maria V.en_US
dc.contributor.authorAntonio-Nkondjio, Christopheen_US
dc.contributor.authorNdo, Cyrilleen_US
dc.contributor.authorWeill, Myleneen_US
dc.contributor.authorSimard, Frédéricen_US
dc.contributor.authorSharakhov, Igor V.en_US
dc.date.accessioned2017-01-05T22:17:39Z
dc.date.available2017-01-05T22:17:39Z
dc.date.issued2011-10-19en_US
dc.identifier.citationParasites & Vectors. 2011 Oct 19;4(1):202
dc.identifier.issn1756-3305en_US
dc.identifier.urihttp://hdl.handle.net/10919/73965
dc.description.abstractBackground Anopheles nili is a major vector of malaria in the humid savannas and forested areas of sub-Saharan Africa. Understanding the population genetic structure and evolutionary dynamics of this species is important for the development of an adequate and targeted malaria control strategy in Africa. Chromosomal inversions and microsatellite markers are commonly used for studying the population structure of malaria mosquitoes. Physical mapping of these markers onto the chromosomes further improves the toolbox, and allows inference on the demographic and evolutionary history of the target species. Results Availability of polytene chromosomes allowed us to develop a map of microsatellite markers and to study polymorphism of chromosomal inversions. Nine microsatellite markers were mapped to unique locations on all five chromosomal arms of An. nili using fluorescent in situ hybridization (FISH). Probes were obtained from 300-483 bp-long inserts of plasmid clones and from 506-559 bp-long fragments amplified with primers designed using the An. nili genome assembly generated on an Illumina platform. Two additional loci were assigned to specific chromosome arms of An. nili based on in silico sequence similarity and chromosome synteny with Anopheles gambiae. Three microsatellites were mapped inside or in the vicinity of the polymorphic chromosomal inversions 2Rb and 2Rc. A statistically significant departure from Hardy-Weinberg equilibrium, due to a deficit in heterozygotes at the 2Rb inversion, and highly significant linkage disequilibrium between the two inversions, were detected in natural An. nili populations collected from Burkina Faso. Conclusions Our study demonstrated that next-generation sequencing can be used to improve FISH for microsatellite mapping in species with no reference genome sequence. Physical mapping of microsatellite markers in An. nili showed that their cytological locations spanned the entire five-arm complement, allowing genome-wide inferences. The knowledge about polymorphic inversions and chromosomal locations of microsatellite markers has been useful for explaining differences in genetic variability across loci and significant differentiation observed among natural populations of An. nili.
dc.format.extent? - ? (10) page(s)en_US
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.publisherBiomed Central Ltden_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000297281900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectParasitologyen_US
dc.subjectPARASITOLOGYen_US
dc.subjectChromosome inversionsen_US
dc.subjectgenome sequenceen_US
dc.subjectmalaria vectoren_US
dc.subjectmicrosatellite markersen_US
dc.subjectpopulation structureen_US
dc.subjectMICROARRAY-BASED ANALYSISen_US
dc.subjectPLASMODIUM-FALCIPARUMen_US
dc.subjectTEMPORAL STABILITYen_US
dc.subjectCULICIDAE LARVAEen_US
dc.subjectGAMBIAE COMPLEXen_US
dc.subjectSARCOPTES MITEen_US
dc.subjectWEST-AFRICAen_US
dc.subjectCAMEROONen_US
dc.subjectTRANSMISSIONen_US
dc.subjectFUNESTUSen_US
dc.titleImproving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomesen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.rights.holderAshley Peery et al.; licensee BioMed Central Ltd.
dc.title.serialPARASITES & VECTORSen_US
dc.identifier.doihttps://doi.org/10.1186/1756-3305-4-202
dc.identifier.volume4en_US
dc.type.dcmitypeText
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomology
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences


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Creative Commons Attribution 4.0 International
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