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dc.contributor.authorHall, Andrew Brantleyen_US
dc.contributor.authorTimoshevskiy, Vladimir A.en_US
dc.contributor.authorSharakhova, Maria V.en_US
dc.contributor.authorJiang, Xiaofangen_US
dc.contributor.authorBasu, Sanjayen_US
dc.contributor.authorAnderson, Michelle A. E.en_US
dc.contributor.authorHu, Wanqien_US
dc.contributor.authorSharakhov, Igor V.en_US
dc.contributor.authorAdelman, Zach N.en_US
dc.contributor.authorTu, Zhijianen_US
dc.date.accessioned2017-01-05T23:43:18Z
dc.date.available2017-01-05T23:43:18Z
dc.date.issued2014-01-01en_US
dc.identifier.issn1759-6653en_US
dc.identifier.urihttp://hdl.handle.net/10919/73979
dc.description.abstractThe preservation of a homomorphic sex-determining chromosome in some organisms without transformation into a heteromorphic sex chromosome is a long-standing enigma in evolutionary biology. A dominant sex-determining locus (or M-locus) in an undifferentiated homomorphic chromosome confers the male phenotype in the yellow fever mosquito Aedes aegypti. Genetic evidence suggests that the M-locus is in a nonrecombining region. However, the molecular nature of the M-locus has not been characterized. Using a recently developed approach based on Illumina sequencing of male and female genomic DNA, we identified a novel gene, myo-sex, that is present almost exclusively in the male genome but can sporadically be found in the female genome due to recombination. For simplicity, we define sequences that are primarily found in the male genome as male-biased. Fluorescence in situ hybridization (FISH) on A. aegypti chromosomes demonstrated that the myo-sex probe localized to region 1q21, the established location of theM-locus.Myo-sex is a duplicated myosin heavy chain gene that is highly expressed in the pupa and adult male.Myo-sex shares 83% nucleotide identity and 97% amino acid identity with its closest autosomal paralog, consistent with ancient duplication followed by strong purifying selection. Compared with males, myo-sex is expressed at very low levels in the females that acquired it, indicating that myo-sexmay be sexually antagonistic. This study establishes a framework to discover male-biased sequences within a homomorphic sex-determining chromosome and offers new insights into the evolutionary forces that have impeded the expansion of the nonrecombining M-locus in A. aegypti.en
dc.format.extent179 - 191 (13) page(s)en_US
dc.languageEnglishen_US
dc.publisherOxford Univ Pressen_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000331289100015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 3.0 Unported (CC BY 3.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/*
dc.subjectEvolutionary Biologyen_US
dc.subjectGenetics & Heredityen_US
dc.subjectEVOLUTIONARY BIOLOGYen_US
dc.subjectGENETICS & HEREDITYen_US
dc.subjectsex-determinationen_US
dc.subjectsex chromosomesen_US
dc.subjectchromosome quotienten_US
dc.subjectmyo-sexen_US
dc.subjectnonrecombining regionen_US
dc.subjectsexual antagonismen_US
dc.subjectYELLOW-FEVER MOSQUITOen_US
dc.subjectSILK FIBROIN GENEen_US
dc.subjectY-CHROMOSOMEen_US
dc.subjectLINKAGE MAPen_US
dc.subjectCULEX-PIPIENSen_US
dc.subjectHUMAN GENOMEen_US
dc.subjectEVOLUTIONen_US
dc.subjectDROSOPHILAen_US
dc.subjectANOPHELESen_US
dc.subjectSEQUENCEen_US
dc.titleInsights into the Preservation of the Homomorphic Sex-Determining Chromosome of Aedes aegypti from the Discovery of a Male-Biased Gene Tightly Linked to the M-Locusen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.rights.holderThe Author(s)en_US
dc.title.serialGENOME BIOLOGY AND EVOLUTIONen_US
dc.identifier.doihttps://doi.org/10.1093/gbe/evu002
dc.identifier.volume6en_US
dc.identifier.issue1en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistry
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomology
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences


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Creative Commons Attribution 3.0 Unported (CC BY 3.0)
License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)