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dc.contributor.authorJiang, X.en
dc.contributor.authorPeery, A.en
dc.contributor.authorHall, B.en
dc.contributor.authorSharma, A.en
dc.contributor.authorChen, X.-G.en
dc.contributor.authorWaterhouse, R. M.en
dc.contributor.authorKomissarov, A.en
dc.contributor.authorRiehle, M. M.en
dc.contributor.authorShouche, Y.en
dc.contributor.authorSharakhova, Maria V.en
dc.contributor.authorLawson, D.en
dc.contributor.authorPakpour, Nazzyen
dc.contributor.authorArensburger, P.en
dc.contributor.authorDavidson, V. L. M.en
dc.contributor.authorEiglmeier, K.en
dc.contributor.authorEmrich, S.en
dc.contributor.authorGeorge, P.en
dc.contributor.authorKennedy, R. C.en
dc.contributor.authorMane, S. P.en
dc.contributor.authorMaslen, G.en
dc.contributor.authorOringanje, C.en
dc.contributor.authorQi, Y.en
dc.contributor.authorSettlage, Robert E.en
dc.contributor.authorTojo, M.en
dc.contributor.authorTubio, J. M. C.en
dc.contributor.authorUnger, Maria F.en
dc.contributor.authorWang, B.en
dc.contributor.authorVernick, K. D.en
dc.contributor.authorRibeiro, J. C.en
dc.contributor.authorJames, A. A.en
dc.contributor.authorMichel, K.en
dc.contributor.authorRiehle, M. A.en
dc.contributor.authorLuckhart, Shirleyen
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.authorTu, Zhijian Jakeen
dc.date.accessioned2017-01-05T23:44:30Zen
dc.date.available2017-01-05T23:44:30Zen
dc.date.issued2014-01-01en
dc.identifier.issn1465-6906en
dc.identifier.urihttp://hdl.handle.net/10919/73982en
dc.description.abstractBackground: Anopheles stephensi is the key vector of malaria throughout the Indian subcontinent and Middle East and an emerging model for molecular and genetic studies of mosquito-parasite interactions. The type form of the species is responsible for the majority of urban malaria transmission across its range. Results: Here, we report the genome sequence and annotation of the Indian strain of the type form of An. stephensi. The 221 Mb genome assembly represents more than 92% of the entire genome and was produced using a combination of 454, Illumina, and PacBio sequencing. Physical mapping assigned 62% of the genome onto chromosomes, enabling chromosome-based analysis. Comparisons between An. stephensi and An. gambiae reveal that the rate of gene order reshuffling on the X chromosome was three times higher than that on the autosomes. An. stephensi has more heterochromatin in pericentric regions but less repetitive DNA in chromosome arms than An. gambiae. We also identify a number of Y-chromosome contigs and BACs. Interspersed repeats constitute 7.1% of the assembled genome while LTR retrotransposons alone comprise more than 49% of the Y contigs. RNA-seq analyses provide new insights into mosquito innate immunity, development, and sexual dimorphism. Conclusions: The genome analysis described in this manuscript provides a resource and platform for fundamental and translational research into a major urban malaria vector. Chromosome-based investigations provide unique perspectives on Anopheles chromosome evolution. RNA-seq analysis and studies of immunity genes offer new insights into mosquito biology and mosquito-parasite interactions.en
dc.format.extent? - ? (18) page(s)en
dc.languageEnglishen
dc.publisherBiomed Central Ltden
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000346604700012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBiotechnology & Applied Microbiologyen
dc.subjectGenetics & Heredityen
dc.subjectPLASMODIUM-FALCIPARUMen
dc.subjectCHROMOSOMAL REARRANGEMENTen
dc.subjectINVERSION POLYMORPHISMSen
dc.subjectNUCLEAR LAMINSen
dc.subjectAEDES-AEGYPTIen
dc.subjectWEB SERVERen
dc.subjectIN-SILICOen
dc.subjectRNA-SEQen
dc.subjectGENEen
dc.subjectEVOLUTIONen
dc.titleGenome analysis of a major urban malaria vector mosquito, Anopheles stephensien
dc.typeArticle - Refereeden
dc.description.versionPublished versionen
dc.rights.holderThe Author(s)en
dc.contributor.departmentBiological Systems Engineeringen
dc.title.serialGENOME BIOLOGYen
dc.identifier.doihttps://doi.org/10.1186/s13059-014-0459-2en
dc.identifier.volume15en
dc.identifier.issue9en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International