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dc.contributor.authorRallos, Lynn Esther E.en
dc.contributor.authorBaudoin, Antonius B.en
dc.date.accessioned2017-01-06T01:31:48Zen
dc.date.available2017-01-06T01:31:48Zen
dc.date.issued2016-02-03en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/10919/73983en
dc.description.abstractDemethylation inhibitors (DMIs) have been an important tool in the management of grapevine powdery mildew caused by Erysiphe necator. Long-term, intensive use of DMIs has resulted in reduced sensitivity in field populations. To further characterize DMI resistance and understand resistance mechanisms in this pathogen, we investigated the cyp51 sequence of 24 single-spored isolates from Virginia and surrounding states and analyzed gene expression in isolates representing a wide range of sensitivity. Two cyp51 alleles were found with respect to the 136th codon of the predicted EnCYP51 sequence: the wildtype (TAT) and the mutant (TTT), which results in the known Y136F amino acid change. Some isolates possessed both alleles, demonstrating gene duplication or increased gene copy number and possibly a requirement for at least one mutant copy of CYP51 for resistance. Cyp51 was over-expressed 1.4- to 19-fold in Y136F-mutant isolates. However, the Y136F mutation was absent in one isolate with moderate to high resistance factor. Two additional synonymous mutations were detected as well, one of which, A1119C was present only in isolates with high cyp51 expression. Overall, our results indicate that at least two mechanisms, cyp51 over-expression and the known target-site mutation in CYP51, contribute to resistance in E. necator, and may be working in conjunction with each other.en
dc.format.extent? - ? (18) page(s)en
dc.languageEnglishen
dc.publisherPLOSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000369550200078&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMultidisciplinary Sciencesen
dc.subjectScience & Technology - Other Topicsen
dc.subjectPOWDERY MILDEW FUNGUSen
dc.subjectDEMETHYLATION INHIBITOR FUNGICIDESen
dc.subject14-ALPHA-DEMETHYLASE GENE CYP51en
dc.subjectUNCINULA-NECATORen
dc.subjectSTEROL DEMETHYLATIONen
dc.subjectMONILINIA-FRUCTICOLAen
dc.subjectMYCOSPHAERELLA-GRAMINICOLAen
dc.subjectDIFFERENTIAL SENSITIVITYen
dc.subjectASPERGILLUS-FUMIGATUSen
dc.subjectFUSARIUM-GRAMINEARUMen
dc.titleCo-Occurrence of Two Allelic Variants of CYP51 in Erysiphe necator and Their Correlation with Over-Expression for DMI Resistanceen
dc.typeArticle - Refereeden
dc.description.versionPublished (Publication status)en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.title.serialPLOS ONEen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0148025en
dc.identifier.volume11en
dc.identifier.issue2en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Plant Pathology, Physiology, & Weed Scienceen
pubs.organisational-group/Virginia Tech/All T&R Facultyen


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International