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dc.contributor.authorCiupe, Mihaela Stancaen_US
dc.contributor.authorDevlin, Blythe H.en_US
dc.contributor.authorMarkert, Mary L.en_US
dc.contributor.authorKepler, Thomas B.en_US
dc.date.accessioned2017-01-22T01:57:35Z
dc.date.available2017-01-22T01:57:35Z
dc.date.issued2013-08-06en_US
dc.identifier.citationBMC Immunology. 2013 Aug 06;14(1):35
dc.identifier.issn1471-2172en_US
dc.identifier.urihttp://hdl.handle.net/10919/74402
dc.description.abstractBackground T-cell receptor diversity correlates with immune competency and is of particular interest in patients undergoing immune reconstitution. Spectratyping generates data about T-cell receptor CDR3 length distribution for each BV gene but is technically complex. Flow cytometry can also be used to generate data about T-cell receptor BV gene usage, but its utility has not been compared to or tested in combination with spectratyping. Results Using flow cytometry and spectratype data, we have defined a divergence metric that quantifies the deviation from normal of T-cell receptor repertoire. We have shown that the sample size is a sensitive parameter in the predicted flow divergence values, but not in the spectratype divergence values. We have derived two ways to correct for the measurement bias using mathematical and statistical approaches and have predicted a lower bound in the number of lymphocytes needed when using the divergence as a substitute for diversity. Conclusions Using both flow cytometry and spectratyping of T-cells, we have defined the divergence measure as an indirect measure of T-cell receptor diversity. We have shown the dependence of the divergence measure on the sample size before it can be used to make predictions regarding the diversity of the T-cell receptor repertoire.
dc.format.extent? - ? (12) page(s)en_US
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.publisherBiomed Central Ltden_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000322999700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectImmunologyen_US
dc.subjectIMMUNOLOGYen_US
dc.subjectREPERTOIRE DIVERSITYen_US
dc.subjectTHYMUS TRANSPLANTATIONen_US
dc.subjectDIGEORGE-SYNDROMEen_US
dc.subjectRECONSTITUTIONen_US
dc.subjectANTIGENen_US
dc.subjectRECOGNITIONen_US
dc.titleQuantification of total T-cell receptor diversity by flow cytometry and spectratypingen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.rights.holderStanca M Ciupe et al.; licensee BioMed Central Ltd.
dc.title.serialBMC IMMUNOLOGYen_US
dc.identifier.doihttps://doi.org/10.1186/1471-2172-14-35
dc.identifier.volume14en_US
dc.type.dcmitypeText
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Science
pubs.organisational-group/Virginia Tech/Science/COS T&R Faculty
pubs.organisational-group/Virginia Tech/Science/Mathematics


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Creative Commons Attribution 4.0 International
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