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dc.contributor.authorYuan, H.en_US
dc.contributor.authorMiller, J. H.en_US
dc.contributor.authorAbu-Reesh, I. M.en_US
dc.contributor.authorPruden, Amyen_US
dc.contributor.authorHe, Z.en_US
dc.date.accessioned2017-02-14T21:52:18Z
dc.date.available2017-02-14T21:52:18Z
dc.date.issued2016-11-01en_US
dc.identifier.issn0048-9697en_US
dc.identifier.urihttp://hdl.handle.net/10919/75036
dc.description.abstractAnaerobic biotechnologies can effectively remove antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), but there is a need to better understand the mechanisms. Here we employ bioelectrochemical systems (BES) as a platform to investigate the fate of a native tetracycline and sulfonamide-resistant Escherichia coli strain and its ARGs. The E. coli strain carrying intI1, sulI and tet(E) was isolated from domestic wastewater and dosed into a tubular BES. The BES was first operated as a microbial fuel cell (MFC), with aeration in the cathode, which resulted in enhanced removal of E. coli and ARGs by ~ 2 log (i.e., order of magnitude) when switched from high current to open circuit operation mode. The BES was then operated as a microbial electrolysis cell (MEC) to exclude the effects of oxygen diffusion, and the removal of E. coli and ARGs during the open circuit configuration was again 1–2 log higher than that at high current mode. Significant correlations of E. coli vs. current (R2 = 0.73) and ARGs vs. E. coli (R2 ranged from 0.54 to 0.87), and the fact that the BES substrate contained no electron acceptors, implied that the persistence of the E. coli and its ARGs was determined by the availability of indigenous electron acceptors in the BES, i.e., the anode electrode or the electron shuttles generated by the exoelectrogens. Subsequent experiments with pure-culture tetracycline and sulfonamide-resistant E. coli being incubated in a two-chamber MEC and serum bottles demonstrated that the E. coli could survive by respiring anode electrode and/or electron shuttles released by exoelectrogens, and ARGs persisted with their host E. coli.en
dc.format.extent1587 - 1594 (8) page(s)en_US
dc.languageEnglishen_US
dc.publisherElsevier Science Bven_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000382269000154&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectEnvironmental Sciencesen_US
dc.subjectEnvironmental Sciences & Ecologyen_US
dc.subjectAntibiotic resistant bacteriaen_US
dc.subjectAntibiotic resistant genesen_US
dc.subjectBioelectrochemical systemsen_US
dc.subjectElectron acceptorsen_US
dc.subjectWASTE-WATER TREATMENTen_US
dc.subjectMICROBIAL FUEL-CELLSen_US
dc.subjectTETRACYCLINE RESISTANCEen_US
dc.subjectMOLECULAR SIGNATURESen_US
dc.subjectDESALINATION CELLSen_US
dc.subjectTREATMENT PLANTSen_US
dc.subjectGROWTH-RATEen_US
dc.subjectTECHNOLOGYen_US
dc.subjectSYSTEMSen_US
dc.subjectRIVERen_US
dc.titleEffects of electron acceptors on removal of antibiotic resistant Escherichia coli, resistance genes and class 1 integrons under anaerobic conditionsen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.contributor.departmentCivil and Environmental Engineeringen_US
dc.title.serialSCIENCE OF THE TOTAL ENVIRONMENTen_US
dc.identifier.doihttps://doi.org/10.1016/j.scitotenv.2016.07.002
dc.identifier.volume569en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Engineering
pubs.organisational-group/Virginia Tech/Engineering/Civil & Environmental Engineering
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Faculty


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