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dc.contributor.authorGarner, Emilyen_US
dc.contributor.authorWallace, Joshua S.en_US
dc.contributor.authorArango-Argoty, Gustavoen_US
dc.contributor.authorWilkinson, Caitlinen_US
dc.contributor.authorFahrenfeld, Nicoleen_US
dc.contributor.authorHeath, Lenwood S.en_US
dc.contributor.authorZhang, Liqingen_US
dc.contributor.authorArabi, Mazdaken_US
dc.contributor.authorAga, Diana S.en_US
dc.contributor.authorPruden, Amyen_US
dc.date.accessioned2017-03-08T19:40:30Z
dc.date.available2017-03-08T19:40:30Z
dc.date.issued2016-12-05en_US
dc.identifier.issn2045-2322en_US
dc.identifier.urihttp://hdl.handle.net/10919/75499
dc.description.abstractRecord-breaking floods in September 2013 caused massive damage to homes and infrastructure across the Colorado Front Range and heavily impacted the Cache La Poudre River watershed. Given the unique nature of this watershed as a test-bed for tracking environmental pathways of antibiotic resistance gene (ARG) dissemination, we sought to determine the impact of extreme flooding on ARG reservoirs in river water and sediment. We utilized high-throughput DNA sequencing to obtain metagenomic profiles of ARGs before and after flooding, and investigated 23 antibiotics and 14 metals as putative selective agents during post-flood recovery. With 277 ARG subtypes identified across samples, total bulk water ARGs decreased following the flood but recovered to near pre-flood abundances by ten months post-flood at both a pristine site and at a site historically heavily influenced by wastewater treatment plants and animal feeding operations. Network analysis of de novo assembled sequencing reads into 52,556 scaffolds identified ARGs likely located on mobile genetic elements, with up to 11 ARGs per plasmid-associated scaffold. Bulk water bacterial phylogeny correlated with ARG profiles while sediment phylogeny varied along the river’s anthropogenic gradient. This rare flood afforded the opportunity to gain deeper insight into factors influencing the spread of ARGs in watersheds.en_US
dc.format.extent? - ? (10) page(s)en_US
dc.languageEnglishen_US
dc.publisherNature Publishing Groupen_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000389190100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMETAL RESISTANCEen_US
dc.subjectSEQUENCING DATAen_US
dc.subjectWASTE-WATERen_US
dc.subjectDATABASEen_US
dc.subjectSOILen_US
dc.subjectTETRACYCLINEen_US
dc.subjectENVIRONMENTen_US
dc.subjectCOSELECTIONen_US
dc.subjectBACTERIAen_US
dc.subjectMANUREen_US
dc.titleMetagenomic profiling of historic Colorado Front Range flood impact on distribution of riverine antibiotic resistance genesen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.title.serialScientific Reportsen_US
dc.identifier.doihttps://doi.org/10.1038/srep38432
dc.identifier.volume6en_US
dc.identifier.orcidHeath, LS [0000-0003-1608-431X]en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Engineering
pubs.organisational-group/Virginia Tech/Engineering/Civil & Environmental Engineering
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Faculty
pubs.organisational-group/Virginia Tech/Engineering/Computer Science
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences


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Creative Commons Attribution 4.0 International (CC BY 4.0)
License: Creative Commons Attribution 4.0 International (CC BY 4.0)