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dc.contributorVirginia Tech
dc.contributor.authorGuo, Weihua
dc.contributor.authorChen, Yingying
dc.contributor.authorWei, Na
dc.contributor.authorFeng, Xueyang
dc.date.accessioned2017-03-28T17:31:56Z
dc.date.available2017-03-28T17:31:56Z
dc.date.issued2016-08-17
dc.identifier.urihttp://hdl.handle.net/10919/76699
dc.description.abstractThe fermentation inhibitors from the pretreatment of lignocellulosic materials, e.g., acetic acid and furfural, are notorious due to their negative effects on the cell growth and chemical production. However, the metabolic reprogramming of the cells under these stress conditions, especially metabolic response for resistance to mixed inhibitors, has not been systematically investigated and remains mysterious. Therefore, in this study, ¹³C metabolic flux analysis (¹³C-MFA), a powerful tool to elucidate the intracellular carbon flux distributions, has been applied to two Saccharomyces cerevisiae strains with different tolerances to the inhibitors under acetic acid, furfural, and mixed (i.e., acetic acid and furfural) stress conditions to unravel the key metabolic responses. By analyzing the intracellular carbon fluxes as well as the energy and cofactor utilization under different conditions, we uncovered varied metabolic responses to different inhibitors. Under acetate stress, ATP and NADH production was slightly impaired, while NADPH tended towards overproduction. Under furfural stress, ATP and cofactors (including both NADH and NADPH) tended to be overproduced. However, under dual-stress condition, production of ATP and cofactors was severely impaired due to synergistic stress caused by the simultaneous addition of two fermentation inhibitors. Such phenomenon indicated the pivotal role of the energy and cofactor utilization in resisting the mixed inhibitors of acetic acid and furfural. Based on the discoveries, valuable insights are provided to improve the tolerance of S. cerevisiae strain and further enhance lignocellulosic fermentation.
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_US
dc.publisherPLOS
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleInvestigate the Metabolic Reprogramming of Saccharomyces cerevisiae for Enhanced Resistance to Mixed Fermentation Inhibitors via ¹³C Metabolic Flux Analysisen_US
dc.typeArticle - Refereeden_US
dc.contributor.departmentBiological Systems Engineeringen_US
dc.title.serialPLOS One
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0161448
dc.identifier.volume11
dc.identifier.issue8
dc.type.dcmitypeTexten_US


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International