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dc.contributor.authorVallabhaneni, Prashanthien_US
dc.date.accessioned2017-04-04T19:49:37Z
dc.date.available2017-04-04T19:49:37Z
dc.date.issued2012-06-22en_US
dc.identifier.otheretd-08092012-090201en_US
dc.identifier.urihttp://hdl.handle.net/10919/76838
dc.description.abstractNon-targeted metabolite profiling by liquid chromatography-mass spectrometry (LC-MS) was used to determine the metabolite responses of Arabidopsis roots to auxin or ethylene. Crosstalk between these hormones regulates many important physiological processes in plants, including the initiation of lateral root formation and the response to gravity. These occur in part through alterations in the levels of flavonoids, specialized plant metabolites that have been shown to act as negative regulators of auxin transport. However, much remains to be learned about auxin and ethylene responses at the level of the metabolome. LC-MS analysis showed that a number of ions changed in response to both hormones in seedling roots. Although classes of specialized metabolites such as flavonols and glucosinolates change in abundance in response to both auxin and ethylene, there was little overlap with regard to the specific metabolites affected. These data will be integrated with information from transcriptomic and proteomic experiments to develop framework models that connect phytohormones and specialized metabolism with specific physiological processes. Previous studies by imaging techniques have shown that flavonols increase in response to both auxin and ethylene in the root elongation zone, but LC-MS showed that flavonols decreased in abundance in response to these hormones. Therefore a method was developed for targeted metabolite profiling of flavonols in individual root tips by flow injection electrospray mass spectrometry. This method uncovered spatial differences in metabolic profiles that were masked in analyses of whole roots or seedlings, and verified that flavonols increase in response to these hormones in root tips.
dc.language.isoen_USen_US
dc.publisherVirginia Techen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectArabidopsisen_US
dc.subjectmetabolite analysisen_US
dc.subjectflow injection electrospray mass spectrometryen_US
dc.subjectauxinen_US
dc.subjectethyleneen_US
dc.titleMetabolomic approaches to understanding the auxin and ethylene response in Arabidopsis rootsen_US
dc.typeThesisen_US
dc.contributor.departmentBiologyen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineBiologyen_US
dc.contributor.committeechairWinkel, Brenda S. J.en_US
dc.contributor.committeememberMuday, Gloria K.en_US
dc.contributor.committeememberTholl, Dorotheaen_US
dc.contributor.committeememberHelm, Richard F.en_US
dc.type.dcmitypeTexten_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08092012-090201/en_US
dc.date.sdate2012-08-09en_US
dc.date.rdate2016-10-18
dc.date.adate2012-08-21en_US


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