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dc.contributor.authorSuh, SeungBeum
dc.contributor.authorTraore, Mahama Aziz
dc.contributor.authorBehkam, Bahareh
dc.date.accessioned2017-05-01T06:42:18Z
dc.date.available2017-05-01T06:42:18Z
dc.date.issued2016-02-18
dc.identifierc6lc00059b.pdf
dc.identifierc6lc00059b1.pdf
dc.identifier.issn1473-0197
dc.identifier.urihttp://hdl.handle.net/10919/77561
dc.description.abstractHigh throughput sorting of micro/nanoparticles of similar sizes is of significant interest in many biological and chemical applications. In this work, we report a simple and cost-effective sorting technique for separation of similarly-sized particles of dissimilar surface properties within a diffusion-based microfluidic platform using chemotaxis in Escherichia coli bacteria. Differences in surface chemistry of two groups of similarly-sized nanoparticles in a mixture were exploited to selectively assemble one particle group onto motile E. coli, through either specific or non-specific adhesion, and separate them from the remaining particle group via chemotaxis of the attached bacteria. To enable optimal operation of the sorting platform, the chemotaxis behavior of E. coli bacteria in response to casamino acids, the chemoeffector of choice was first characterized. The chemical concentration gradient range within which the bacteria exhibit a positive chemotactic response was found to be within 0.25 × 10−7–1.0 × 10−3 g ml−1 mm−1. We demonstrate that at the optimum concentration gradient of 5.0 × 10−4 g ml−1 mm−1, a sorting efficiency of up to 81% at a throughput of 2.4 × 105 particles per min can be achieved. Sensitivity of the sorting efficiency to the adhesion mechanism and particle size in the range of 320–1040 nm was investigated.
dc.format.extent1254-1260
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofRoyal Society of Chemistry Gold Open Access - 2016
dc.rightsCC BY-NC 3.0
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.titleBacterial chemotaxis-enabled autonomous sorting of nanoparticles of comparable sizes
dc.typeArticle - Refereed
dc.rights.holderSuh, SeungBeum
dc.rights.holderTraore, Mahama A.
dc.rights.holderBehkam, Bahareh
dc.contributor.departmentVirginia Tech. Department of Mechanical Engineering
dc.contributor.departmentVirginia Tech. School of Biomedical Engineering and Sciences
dc.title.serialLab on a Chip
dc.identifier.doihttps://doi.org/10.1039/c6lc00059b
dc.identifier.volume16
dc.identifier.issue7
dc.type.dcmitypeText
dc.type.dcmitypeDataset
dc.identifier.eissn1473-0189


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License: CC BY-NC 3.0