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dc.contributor.authorVaughan, Martha M.en_US
dc.contributor.authorTholl, Dorotheaen_US
dc.contributor.authorTokuhisa, James G.en_US
dc.date.accessioned2017-05-26T19:19:38Z
dc.date.available2017-05-26T19:19:38Z
dc.date.issued2011-03-10en_US
dc.identifier.citationPlant Methods. 2011 Mar 10;7(1):5
dc.identifier.issn1746-4811en_US
dc.identifier.urihttp://hdl.handle.net/10919/77864
dc.description.abstractBackground Plant defense against herbivory has been studied primarily in aerial tissues. However, complex defense mechanisms have evolved in all parts of the plant to combat herbivore attack and these mechanisms are likely to differ in the aerial and subterranean environment. Research investigating defense responses belowground has been hindered by experimental difficulties associated with the accessibility and quality of root tissue and the lack of bioassays using model plants with altered defense profiles. Results We have developed an aeroponic culture system based on a calcined clay substrate that allows insect herbivores to feed on plant roots while providing easy recovery of the root tissue. The culture method was validated by a root-herbivore system developed for Arabidopsis thaliana and the herbivore Bradysia spp. (fungus gnat). Arabidopsis root mass obtained from aeroponically grown plants was comparable to that from other culture systems, and the plants were morphologically normal. Bradysia larvae caused considerable root damage resulting in reduced root biomass and water absorption. After feeding on the aeroponically grown root tissue, the larvae pupated and emerged as adults. Root damage of mature plants cultivated in aeroponic substrate was compared to that of Arabidopsis seedlings grown in potting mix. Seedlings were notably more susceptible to Bradysia feeding than mature plants and showed decreased overall growth and survival rates. Conclusions A root-herbivore system consisting of Arabidopsis thaliana and larvae of the opportunistic herbivore Bradysia spp. has been established that mimics herbivory in the rhizosphere. Bradysia infestation of Arabidopsis grown in this culture system significantly affects plant performance. The culture method will allow simple profiling and in vivo functional analysis of root defenses such as chemical defense metabolites that are released in response to belowground insect attack.
dc.format.extent? - ? (10) page(s)en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherBiomed Central Ltden_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000288815900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBiochemical Research Methodsen_US
dc.subjectPlant Sciencesen_US
dc.subjectBiochemistry & Molecular Biologyen_US
dc.subjectPLANT SCIENCESen_US
dc.subjectBELOW-GROUND HERBIVORYen_US
dc.subjectPLANT DEFENSEen_US
dc.subjectFUNGUS GNATSen_US
dc.subjectENTOMOPATHOGENIC NEMATODESen_US
dc.subjectCOPROPHILA DIPTERAen_US
dc.subjectINDUCED RESPONSESen_US
dc.subjectBRADYSIAen_US
dc.subjectSCIARIDAEen_US
dc.subjectTOLERANCEen_US
dc.subjectDIVERSITYen_US
dc.titleAn aeroponic culture system for the study of root herbivory on Arabidopsis thalianaen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.rights.holderMartha M Vaughan et al.; licensee BioMed Central Ltd.en
dc.contributor.departmentBiological Sciencesen_US
dc.contributor.departmentSchool of Plant and Environmental Sciencesen_US
dc.title.serialPLANT METHODSen_US
dc.identifier.doihttps://doi.org/10.1186/1746-4811-7-5
dc.identifier.volume7en_US
dc.identifier.orcidTholl, D [0000-0003-2636-6345]en_US
dc.identifier.orcidTokuhisa, JG [0000-0002-3834-5149]en_US
dc.type.dcmitypeText
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Science
pubs.organisational-group/Virginia Tech/Science/Biological Sciences
pubs.organisational-group/Virginia Tech/Science/COS T&R Faculty
pubs.organisational-group/Virginia Tech/University Research Institutes
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Faculty


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International