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dc.contributor.authorWebster, Kelly Eileenen_US
dc.date.accessioned2017-06-23T08:00:43Z
dc.date.available2017-06-23T08:00:43Z
dc.date.issued2017-06-22en_US
dc.identifier.othervt_gsexam:11958en_US
dc.identifier.urihttp://hdl.handle.net/10919/78244
dc.description.abstractWhile machine perfusion of explanted kidneys is theoretically superior to standard cold storage, it may damage potential transplants unless machine-associated swelling is controlled. This thesis presents the effects of perfusate tonicity on renal swelling during hypothermic machine perfusion. Phosphate buffered solution (PBS) and PBS supplemented with 5% w/v mannitol were used as isotonic (289 mOsm/kg) and hypertonic (568 mOsm/kg) perfusates, respectively. Porcine kidney pairs were procured then flushed and machine perfused; the right and left kidneys were assigned opposite perfusates. An experimental methodology was developed to image porcine kidneys undergoing hypothermic machine perfusion (5 deg C) for 15 minutes followed by 120 minutes without perfusion to quantify surface displacement (renal swelling) with digital image correlation (DIC). Surface displacement and size (thickness) were compared between the right and left kidneys of each pair. In addition, discharged renal fluids (i.e., filtrate and venous outflow) and biopsies were collected. On average, kidneys perfused with the mannitol solution were smaller in size than the kidneys perfused with PBS (p < 0.05) at the start and end of each experiment; however, there was no significant difference between the renal sizes at the end of the 15 minute perfusion interval (p > 0.05). Thus, hypertonic and isotonic perfusates yielded different renal swelling outcomes (i.e., physical size and surface displacement), which suggests that perfusate tonicity influences renal swelling. These experiments are the first time ex vivo renal surface displacement measurements have been collected during machine perfusion.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsThis item is protected by copyright and/or related rights. Some uses of this item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en_US
dc.subjectTransplanten_US
dc.subjectKidneyen_US
dc.subjectEdemaen_US
dc.subjectMachine Perfusionen_US
dc.subjectDigital Image Correlationen_US
dc.subjectPerfusate Compositionen_US
dc.subjectOsmolalityen_US
dc.subjectTonicityen_US
dc.titleQuantifying Renal Swelling during Machine Perfusion using Digital Image Correlationen_US
dc.typeThesisen_US
dc.contributor.departmentMechanical Engineeringen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineMechanical Engineeringen_US
dc.contributor.committeechairRobertson, John L.en_US
dc.contributor.committeechairJohnson, Blakeen_US
dc.contributor.committeememberDiller, Thomas E.en_US
dc.contributor.committeememberWest, Robert L.en_US


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