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dc.contributor.authorJones, Taylor J.en_US
dc.date.accessioned2017-12-30T07:00:28Z
dc.date.available2017-12-30T07:00:28Z
dc.date.issued2016-07-07en_US
dc.identifier.othervt_gsexam:8232en_US
dc.identifier.urihttp://hdl.handle.net/10919/81460
dc.description.abstractIn order to aid the booming wine industry in the state of Virginia, U.S.A., we developed a series of studies to provide a deeper understanding of the viruses and vectors for management of virus diseases and development of better tools for grapevine virus diagnostics. A statewide survey for 14 different grapevine viruses between 2009 and 2014 was conducted: 721 samples were collected from 116 vineyards in the period. Among the 12 viruses identified, Grapevine leafroll associated virus-3 (GLRaV-3), Grapevine rupestris stem-pitting associated virus (GRSPaV), and Grapevine red blotch-associated virus (GRBaV) were most commonly present. A new real-time PCR method for the detection of the V2 gene of GRBaV was developed. The resulting method takes less time for more accurate diagnostics than conventional PCR. Evaluation of insecticide effectiveness on GLRaV-3 vectors (mealybugs) and the spread of GLRaV-3 were examined: Four trials conducted from 2012 to 2014 revealed that despite successful control of mealybugs, GLRaV-3 is spread at a very rapid rate. A new sampling technique for efficient nucleic acid storage and testing was developed: the nitrocellulose membrane-based method allows simpler extraction of nucleic acid and provides a storage medium that can hold viable RNA/DNA at room temperature for up to 18 months. An investigation of multiple virus-infected vines and the impact of these co-infections on grapevine fruit chemistry was conducted. GLRaV-3, GRBaV, GRSPaV, and co-infections of the 3 all negatively impacted Brix, pH, titratable acidity, and anthocyanin levels.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsThis Item is protected by copyright and/or related rights. Some uses of this Item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en_US
dc.subjectGrapevine leafroll diseaseen_US
dc.subjectGrapevine red blotch associated virusen_US
dc.subjectNitrocellulose Membranesen_US
dc.subjectreal-time PCRen_US
dc.subjectMealybugsen_US
dc.subjectGrapevinesen_US
dc.subjectVirginiaen_US
dc.titleGrapevine Viruses and Associated Vectors in Virginia: Survey, Vector Management, and Development of Efficient Grapevine Virus Testing Methodsen_US
dc.typeDissertationen_US
dc.contributor.departmentPlant Pathology, Physiology, and Weed Scienceen_US
dc.description.degreePh. D.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplinePlant Pathology, Physiology, and Weed Scienceen_US
dc.contributor.committeechairNita, Mizuhoen_US
dc.contributor.committeechairBaudoin, Antonius B.en_US
dc.contributor.committeememberWang, Xiaofengen_US
dc.contributor.committeememberPfeiffer, Douglas G.en_US
dc.contributor.committeememberRayapati, Naidu A.en_US


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