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dc.contributor.authorVu, LTen_US
dc.contributor.authorOrbach, SMen_US
dc.contributor.authorRay, WKen_US
dc.contributor.authorCassin, MEen_US
dc.contributor.authorRajagopalan, Pen_US
dc.contributor.authorHelm, RFen_US
dc.date.accessioned2018-01-08T14:22:03Z
dc.date.available2018-01-08T14:22:03Z
dc.date.issued2017-06-20en_US
dc.identifier.citationProteome Science. 2017 Jun 20;15(1):12
dc.identifier.issn1477-5956en_US
dc.identifier.urihttp://hdl.handle.net/10919/81601
dc.description.abstractBackground Liver models that closely mimic the in vivo microenvironment are useful for understanding liver functions, capabilities, and intercellular communication processes. Three-dimensional (3D) liver models assembled using hepatocytes and liver sinusoidal endothelial cells (LSECs) separated by a polyelectrolyte multilayer (PEM) provide a functional system while also permitting isolation of individual cell types for proteomic analyses. Methods To better understand the mechanisms and processes that underlie liver model function, hepatocytes were maintained as monolayers and 3D PEM-based formats in the presence or absence of primary LSECs. The resulting hepatocyte proteomes, the proteins in the PEM, and extracellular levels of urea, albumin and glucose after three days of culture were compared. Results All systems were ketogenic and found to release glucose. The presence of the PEM led to increases in proteins associated with both mitochondrial and peroxisomal-based β-oxidation. The PEMs also limited production of structural and migratory proteins associated with dedifferentiation. The presence of LSECs increased levels of Phase I and Phase II biotransformation enzymes as well as several proteins associated with the endoplasmic reticulum and extracellular matrix remodeling. The proteomic analysis of the PEMs indicated that there was no significant change after three days of culture. These results are discussed in relation to liver model function. Conclusions Heterotypic cell-cell and cell-ECM interactions exert different effects on hepatocyte functions and phenotypes.
dc.format.extent? - ? (15) page(s)en_US
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.publisherBiomed Central Ltden_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000405227100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBiochemical Research Methodsen_US
dc.subjectBiochemistry & Molecular Biologyen_US
dc.subjectHepatocyteen_US
dc.subjectKetogenesisen_US
dc.subjectLiveren_US
dc.subjectPolyelectrolyte multilayeren_US
dc.subjectProteomicsen_US
dc.subjectCELL-CELL INTERACTIONSen_US
dc.subjectFARNESOID-X-RECEPTORen_US
dc.subjectIN-VITROen_US
dc.subjectGLUCOSE-METABOLISMen_US
dc.subjectNONPARENCHYMAL CELLSen_US
dc.subjectSYSTEMS BIOLOGYen_US
dc.subjectMONOLAYER-CULTURESen_US
dc.subjectCOLLAGEN SANDWICHen_US
dc.subjectDRUG-METABOLISMen_US
dc.subjectCANCER CELLSen_US
dc.titleThe hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironmenten_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.title.serialPROTEOME SCIENCEen_US
dc.identifier.doihttps://doi.org/10.1186/s12953-017-0120-6
dc.identifier.volume15en_US
dc.identifier.orcidHelm, RF [0000-0001-5317-0925]en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistry
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Faculty


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Creative Commons Attribution 4.0 International (CC BY 4.0)
License: Creative Commons Attribution 4.0 International (CC BY 4.0)