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dc.contributor.authorSantolamazza, Federicaen_US
dc.contributor.authorMancini, Emilianoen_US
dc.contributor.authorSimard, Frédéricen_US
dc.contributor.authorQi, Yuminen_US
dc.contributor.authorTu, Zhijianen_US
dc.contributor.authordella Torre, Alessandraen_US
dc.date.accessioned2018-01-15T05:28:29Z
dc.date.available2018-01-15T05:28:29Z
dc.date.issued2008-08-25en_US
dc.identifier.citationMalaria Journal. 2008 Aug 25;7(1):163
dc.identifier.issn1475-2875en_US
dc.identifier.urihttp://hdl.handle.net/10919/81775
dc.description.abstractBackground SINEs (Short INterspersed Elements) are homoplasy-free and co-dominant genetic markers which are considered to represent useful tools for population genetic studies, and could help clarifying the speciation processes ongoing within the major malaria vector in Africa, Anopheles gambiae s.s. Here, we report the results of the analysis of the insertion polymorphism of a nearly 200 bp-long SINE (SINE200) within genome areas of high differentiation (i.e. "speciation islands") of M and S A. gambiae molecular forms. Methods A SINE-PCR approach was carried out on thirteen SINE200 insertions in M and S females collected along the whole range of distribution of A. gambiae s.s. in sub-Saharan Africa. Ten specimens each for Anopheles arabiensis, Anopheles melas, Anopheles quadriannulatus A and 15 M/S hybrids from laboratory crosses were also analysed. Results Eight loci were successfully amplified and were found to be specific for A. gambiae s.s.: 5 on 2L chromosome and one on X chromosome resulted monomorphic, while two loci positioned respectively on 2R (i.e. S200 2R12D) and X (i.e. S200 X6.1) chromosomes were found to be polymorphic. S200 2R12D was homozygote for the insertion in most S-form samples, while intermediate levels of polymorphism were shown in M-form, resulting in an overall high degree of genetic differentiation between molecular forms (Fst = 0.46 p < 0.001) and within M-form (Fst = 0.46 p < 0.001). The insertion of S200 X6.1 was found to be fixed in all M- and absent in all S-specimens. This led to develop a novel easy-to-use PCR approach to straightforwardly identify A. gambiae molecular forms. This novel approach allows to overcome the constraints associated with markers on the rDNA region commonly used for M and S identification. In fact, it is based on a single copy and irreversible SINE200 insertion and, thus, is not subjected to peculiar evolutionary patterns affecting rDNA markers, e.g. incomplete homogenization of the arrays through concerted evolution and/or mixtures of M and S IGS-sequences among the arrays of single chromatids. Conclusion The approach utilized allowed to develop new easy-to-use co-dominant markers for the analysis of genetic differentiation between M and S-forms and opens new perspectives in the study of the speciation process ongoing within A. gambiae.
dc.format.extent? - ? (10) page(s)en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherBiomed Central Ltden_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000259430100002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectInfectious Diseasesen_US
dc.subjectParasitologyen_US
dc.subjectTropical Medicineen_US
dc.subjectGENETIC DIFFERENTIATIONen_US
dc.subjectTRANSPOSABLE ELEMENTSen_US
dc.subjectPOPULATION-STRUCTUREen_US
dc.subjectWEST-AFRICAen_US
dc.subjectMOSQUITOen_US
dc.subjectCOMPLEXen_US
dc.subjectARABIENSISen_US
dc.subjectSEQUENCEen_US
dc.subjectGENOMEen_US
dc.subjectS.Sen_US
dc.titleInsertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular formsen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.title.serialMALARIA JOURNALen_US
dc.identifier.doihttps://doi.org/10.1186/1475-2875-7-163
dc.identifier.volume7en_US
dc.type.dcmitypeText
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistry
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Faculty


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