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dc.contributor.authorMiao, Jen_US
dc.contributor.authorFrazier, Ten_US
dc.contributor.authorHuang, Len_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorZhao, Ben_US
dc.coverage.spatialSwitzerlanden_US
dc.date.accessioned2018-01-23T13:33:54Z
dc.date.available2018-01-23T13:33:54Z
dc.date.issued2016en_US
dc.identifier.issn1664-462Xen_US
dc.identifier.urihttp://hdl.handle.net/10919/81896
dc.description.abstractSwitchgrass is one of the most promising energy crops and only recently has been employed for biofuel production. The draft genome of switchgrass was recently released; however, relatively few switchgrass genes have been functionally characterized. CENH3, the major histone protein found in centromeres, along with canonical H3 and other histones, plays an important role in maintaining genome stability and integrity. Despite their importance, the histone H3 genes of switchgrass have remained largely uninvestigated. In this study, we identified 17 putative switchgrass histone H3 genes in silico. Of these genes, 15 showed strong homology to histone H3 genes including six H3.1 genes, three H3.3 genes, four H3.3-like genes and two H3.1-like genes. The remaining two genes were found to be homologous to CENH3. RNA-seq data derived from lowland cultivar Alamo and upland cultivar Dacotah allowed us to identify SNPs in the histone H3 genes and compare their differential gene expression. Interestingly, we also found that overexpression of switchgrass histone H3 and CENH3 genes in N. benthamiana could trigger cell death of the transformed plant cells. Localization and deletion analyses of the histone H3 and CENH3 genes revealed that nuclear localization of the N-terminal tail is essential and sufficient for triggering the cell death phenotype. Our results deliver insight into the mechanisms underlying the histone-triggered cell death phenotype and provide a foundation for further studying the variations of the histone H3 and CENH3 genes in switchgrass.en_US
dc.format.extent979 - ? page(s)en_US
dc.languageengen_US
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/27462323en_US
dc.subjectAgrobacterium-mediated transient assayen_US
dc.subjectCENH3en_US
dc.subjectPanicum virgatum L.en_US
dc.subjectcell deathen_US
dc.subjecthistone H3en_US
dc.titleIdentification and Characterization of Switchgrass Histone H3 and CENH3 Genes.en_US
dc.typeArticle - Refereed
dc.description.versionPublished online (Publication status)en_US
dc.title.serialFront Plant Scien_US
dc.identifier.doihttps://doi.org/10.3389/fpls.2016.00979
dc.identifier.volume7en_US
dcterms.dateAccepted2016-06-21en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Horticulture
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/University Research Institutes
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Faculty


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