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dc.contributor.authorTrahan, Richard Angellasen_US
dc.date.accessioned2018-06-09T08:00:48Z
dc.date.available2018-06-09T08:00:48Z
dc.date.issued2018-06-08en_US
dc.identifier.othervt_gsexam:15611en_US
dc.identifier.urihttp://hdl.handle.net/10919/83501
dc.description.abstractThe objective was to compare the effects of three corticosteroids at various equimolar concentrations on equine articular explant co-cultures in an inflammatory environment. Synovial and osteochondral explant co-cultures from 6 equine cadavers were exposed to IL-1β (10 ng/mL) and various concentrations (10-4, 10-7, or 10-10 M) of MPA, TA, IPA. Concentrations of PGE2, MMP-13, LDH, and GAG in media were determined at 48 and 96 hours. Results indicated wells with low concentrations of MPA (10-7 and 10-10 M at 48 and 96 hours), TA (10-7 M at 48 hours and 10-7 and 10-10 M at 48 and 96 hours), and IPA (10-10 M at 48 hours) had significantly less PGE2 than positive control samples. Groups with low concentrations (10-7 and 10-10 M) of MPA and TA had significantly less PGE2 than the highest concentration (10-4 M) at 48 hours. Significantly less MMP-13 was detected for all concentrations of MPA, TA, and IPA at 96 hours. The LDH assay results indicated cytotoxicity only for samples treated with IPA at 10-4 M at 48 and 96 hours. GAG was significantly lower for samples treated with TA 10-7 M at 48 hours and MPA 10-10 M at 96 hours versus positive controls. These findings suggest corticosteroids at low concentrations mitigated the inflammatory and catabolic effects of IL-1β to a greater extent than high concentrations. Effects of IPA and MPA were similar to TA at clinically relevant low equimolar concentrations.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectosteoarthritisen_US
dc.subjectcorticosteroiden_US
dc.subjectosteochondral explanten_US
dc.subjectisoflupredone acetateen_US
dc.titleEffects of Three Corticosteroids on Equine Articular Cocultures In Vitroen_US
dc.typeThesisen_US
dc.contributor.departmentBiomedical and Veterinary Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineBiomedical and Veterinary Sciencesen_US
dc.contributor.committeechairByron, Christopher R.en_US
dc.contributor.committeememberDahlgren, Linda A.en_US
dc.contributor.committeememberPleasant, Robert S.en_US


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